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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 4

sp. nov., isolated from dried rice husk No Access

Abstract

Strain RHs26 is an aerobic, Gram-stain-negative, non-flagellated and rod- or filamentous-shaped (1.0–1.1×2.3–50 µm) bacterium that was isolated from dried rice husk. It was positive for oxidase and catalase, hydrolysed starch and Tween 80, and weakly hydrolysed CM-cellulose. The strain grew at temperatures between 10 and 37 °C (optimum, 28 °C), in 0–1 % NaCl (optimum, 0 %) and at pH 6.0–9.0 (optimum, pH 7.0–8.0). The predominant membrane fatty acids were summed feature 3 (C 7 and/or C 6), C 5, iso-C and iso-C 3-OH. The major polar lipids were phosphatidylethanolamine, an unidentified aminolipid, two unidentified aminophospholipids and two unidentified lipids. The predominant quinone was menaquinone MK-7. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain RHs26 belongs to the genus , presenting the highest sequence similarity to S7-3-3 (95.8 %). The genomic DNA G+C content of strain RHs26 was 49.5 %. Strain RHs26 showed the highest orthologous average nucleotide identity (OrthoANI) and digital DNA–DNA hybridization (dDDH) values of 76.4 % and 20.0 % with KCTC 52727 while sharing OrthoANI and dDDH values of 74.6 % and 19.2 % with KCTC 52035, the closest relative in the phylogenomic tree. Based on the results of a polyphasic taxonomic study, strain RHs26 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is RHs26 (=JCM 35224=KACC 17318).

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  • Accepted:
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Keyword(s): new species , rice husk , Spirosoma oryzicola and taxonomy
© 2023 The Authors
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2023-04-20
2024-04-25
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Spirosoma oryzicola sp. nov., isolated from dried rice husk
Int J Syst Evol Microbiol 73, 005837 (2023); https://doi.org/10.1099/ijsem.0.005837
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