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Volume 73, Issue 3

sp. nov., a novel marine actinobacterium isolated from seawater in the upper gulf of Thailand No Access

Abstract

An actinobacterium strain, SW21, was isolated from seawater collected in the upper Gulf of Thailand. Cells were Gram-stain-positive, aerobic and rod-shaped. Growth was observed from 15 to 37 °C and at pH 6–8. Maximum NaCl for growth was 14 % (w/v). -Diaminopimelic acid, arabinose, galactose, glucose, rhamnose and ribose were detected in the whole-cell hydrolysate. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside were detected as the phospholipids in the cells. The major menaquinones were MK-9(H) and MK-7(H). The major cellular fatty acids were C, C 9, C and C10-methyl (TBSA). The 16S rRNA gene sequence data supported the assignment of strain SW21 to the genus and showed that KCTC 49383 (98.7 %) was the closest relative. Moreover, the average nucleotide identity- (85.5 %) and digital DNA–DNA hybridization (30.7 %) values between strain SW21 and its closest neighbour were below the threshold values for delineation of a novel species. The combination of genotypic and phenotypic data indicated that strain SW21 is representative of novel species of the genus . The name sp. nov. is proposed for strain SW21. The type strain is SW21 (=TBRC 15691=NBRC 115558).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , actinomycete , Gordonia , Gulf of Thailand and whole-genome sequence analysis
Funding
This study was supported by the:
  • The School of Science, King Mongkut’s Institute of Technology Ladkrabang (Award CW-1-2564-M-001)
    • Principle Award Recipient: RawiratPansomsuay
  • The National Science, Research and Innovation Fund (NSRF) (Award RE-KRIS/FF65/23)
    • Principle Award Recipient: ChittiThawai
© 2023 The Authors
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2023-03-24
2024-04-18
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Gordonia aquimaris sp. nov., a novel marine actinobacterium isolated from seawater in the upper gulf of Thailand
Int J Syst Evol Microbiol 73, 005804 (2023); https://doi.org/10.1099/ijsem.0.005804
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