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Abstract

A novel species in the genus was isolated from soil and plant surfaces in wild cranberry bogs of the Cape Cod National Seashore. is a little-known genus in the family () that is associated with freshwater habitats. As part of a culture-dependent comparative survey of bacteria in wild and cultivated cranberry bogs in Massachusetts, strains MWU14-2217 and MWU14-2470 were characterized for taxonomic placement by phenotypic and genotypic testing, and confirmed by genome comparison with a third isolate, MWU14-2238. Genome analysis methods placed the isolates in the genus with >98.54 % 16S rRNA gene sequence similarity each to the four recognized species, but with a DNA–DNA relatedness of 46 % by digital DNA–DNA hybridization (formula d) and 91.92 % by orthologous average nucleotide identity with with its closest relative. DNA G+C content of the three isolates ranged from 60.11 to 60.28 mol%. The strains are facultatively anaerobic, motile and reduce NO. They are non-pigmented, non-haemolytic on sheep blood agar, grow on mannitol but not on citrate, and do not produce urease or HCN. The predominant fatty acids of both isolates were C and summed Cω7/Cω6. Using a combination of phylogenetic, physiological and phenotypic characterization, isolates MWU14-2217, MWU14-2470 and MWU14-2238 represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is MWU14-2217 (=CCOS 1980=ATCC TSD-261).

Funding
This study was supported by the:
  • Midwestern University
    • Principle Award Recipient: MelindaSchreiber
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-04-03
2025-02-10
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