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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 3

Phylogenomic analysis of the genus and description of sp. nov., sp. nov., subsp. subsp. nov., subsp. subsp. nov., subsp. subsp. nov., subsp. subsp. nov. and subsp. subsp. nov., isolated from floral nectar and insects No Access

Abstract

The genus is one of the most frequent bacterial inhabitants of flowers and a usual member of the insect microbiota worldwide. To date, there is only one publicly available genome, corresponding to the type strain of (8N4), which precludes a detailed analysis of intra-genus phylogenetic relationships. In this study, we obtained draft genomes of the type strains of the other species validly published to date (, and ) and 23 additional isolates of flower and insect origin. Isolate S61, retrieved from the nectar of an sp. flower collected in southern Spain, displayed low average nucleotide identity (ANI) and DNA–DNA hybridization (isDDH) values when compared with other members (≤86.5 and ≤29.8 %, respectively). Similarly, isolate JB07, which was obtained from the floral nectar of plants in Hawaii (USA) had ≤95.7 % ANI and ≤64.1 % isDDH with other isolates. Therefore, our results support the description of two new species for which we propose the names sp. nov. (type strain: S61=NCCB 100789=DSM 111181) and sp. nov. (JB07=NCCB 100888=LMG 32616). Additionally, some and isolates showed isDDH values<79 % with other conspecific isolates, which suggests that these species include subspecies for which we propose the names subsp. subsp. nov. (S256=CECT 8502=LMG 27956), subsp. subsp. nov. (FR72=NCCB 100898=LMG 32786), subsp. subsp. nov. (K24=NCCB 100924=LMG 32785), subsp. subsp. nov. (8N4 = DSM 24150 = LMG 26121) and subsp. subsp. nov. (B1A=NCCB 100810= DSM 111763), respectively. Finally, we present the first phylogenomic analysis of the genus and update the formal description of the species , , and based on new genomic and phenotypic information.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): floral nectar , insect , phylogenomics , Rosenbergiella and taxonomy
Funding
This study was supported by the:
  • Fundación BBVA
    • Principle Award Recipient: Clarade Vega
  • Ministerio de Ciencia e Innovación (Award RYC2018-023847-I)
    • Principle Award Recipient: SergioAlvarez-Perez
  • Deutsche Forschungsgemeinschaft (Award DFG, JU2856/2–2)
    • Principle Award Recipient: ApplicableNot
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005777
2023-03-08
2024-04-20
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Phylogenomic analysis of the genus Rosenbergiella and description of Rosenbergiella gaditana sp. nov., Rosenbergiella metrosideri sp. nov., Rosenbergiella epipactidis subsp. epipactidis subsp. nov., Rosenbergiella epipactidis subsp. californiensis subsp. nov., Rosenbergiella epipactidis subsp. japonicus subsp. nov., Rosenbergiella nectarea subsp. nectarea subsp. nov. and Rosenbergiella nectarea subsp. apis subsp. nov., isolated from floral nectar and insects
Int J Syst Evol Microbiol 73, 005777 (2023); https://doi.org/10.1099/ijsem.0.005777
/content/journal/ijsem/10.1099/ijsem.0.005777
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