1887

Abstract

A novel strain, Bin7N, was isolated from the honey stomach of the honey bee . Cells are Gram-positive, non-motile, non-sporulating, facultative anaerobic and fructose 6-phosphate phosphoketolase-positive. Their optimal growth is at 37 °C in anaerobiosis in MRS (De Man, Rogosa and Sharpe) added with cysteine. The honey bee microbiota was composed of several phylotypes of and . Comparative analysis of 16S rRNA gene sequence similarity revealed that strain Bin7N grouped with species originating from honey bees and was closely related to DSM 20089 (99.67 % similarity). However, the highest average nucleotide identity and digital DNA–DNA hybridization values of 94.88 and 60.6 %, respectively, were obtained with JCM 34586. The DNA G+C content of the type strain is 60.8 mol%. The cell-wall peptidoglycan is of the A4β -Orn–-Asp type. The main cellular fatty acids of strain Bin7N are C ω9, C, C ω7 and C. Phenotypic characterization and genotyping based on the genome sequences clearly show that this strain is distinct from the type strains of the so far recognized species. Thus, sp. nov. (Bin7N=DSM 29108=CCUG 66113) is proposed as novel species.

Funding
This study was supported by the:
  • EU FP7 (Award BEEDOC 244956CP-FP)
    • Principle Award Recipient: TobiasC Olofsson
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2023-03-08
2024-02-26
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