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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 4

sp. nov., isolated from anaerobic fermentation liquid of food waste by high-throughput cultivation No Access

Abstract

Strain CY1518 was isolated from an anaerobic fermentation liquid of food waste treatment plant in Beijing, PR China, and characterized to assess its taxonomy. Cells of CY1518 were Gram-stain-negative, oxidase-negative, catalase-positive and ellipsoidal. Growth occurred at 20–42 °C (optimum, 37 °C), pH 6.0–10.0 (optimum, pH 8) and with 0–6.0 % (w/v) NaCl (optimum, 1.5%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CY1518 belongs to the genus , with the highest sequence similarity to W11-5 (95.97 %), followed by SW127 (95.08%). The similarity between strain CY1518 and other strains of was less than 95 %. The genomic DNA G+C content of strain CY1518 was 60.88 mol%. The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between strain CY1518 and the closely related taxa W11-5 and SW127 were 77.61, 78.03 and 21.2 % and 74.15, 70.02 and 19.3%, respectively. The strain was able to use -serine, Tween 40 and some organic acid compounds for growth. The polar lipids comprised aminophospholipid, diphosphatidylglycerol, glycolipid, an unknown polar lipid, phosphatidylethanolamine, phosphatidylglycerol and phospholipid. The principal fatty acids (>5 %) were C cyclo 8 (36.3%), C (32.3%), C 3-OH (8.3%) and C (7.6%). Based on its phenotypic, genotypic and genomic characteristics, strain CY1518 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is CY1518 (=GDMCC 1.2918=JCM 35120).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Alcanivoracaceae , biogas system , ectoine , food waste and polyphasic taxonomy
Funding
This study was supported by the:
  • University–Industry Collaborative Education Program (Award 202102083002)
    • Principle Award Recipient: GuozhuZhao
  • Fundamental Research Funds for the Central Universities at Beijing Forestry University (Award 2021ZY61)
    • Principle Award Recipient: GuozhuZhao
© 2023 The Authors
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2023-04-24
2025-04-24
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Alcanivorax quisquiliarum sp. nov., isolated from anaerobic fermentation liquid of food waste by high-throughput cultivation
Int J Syst Evol Microbiol 73, 005764 (2023); https://doi.org/10.1099/ijsem.0.005764
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