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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 4

sp. nov., isolated from the rhizosphere of soya bean No Access

Abstract

A Gram-positive, facultatively anaerobic, agar-hydrolytic and rod-shaped bacterium with peritrichous flagellation, designated strain SCIV0701, was isolated from soya bean rhizosphere soil collected from Bazhong, Sichuan Province, PR China and characterized by using polyphasic taxonomy. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCIV0701 belonged to the genus , and showed highest similarity to MX2-3 (97.59 %), M4BSY-1 (97.45 %) and NB5 (97.45 %). The average nucleotide identity values and DNA–DNA hybridization scores between strain SCIV0701 and MX2-3, M4BSY-1 and NB5 were lower than recommended thresholds of 95% and 70 %, respectively, for species delineation. Menaquinone-7 was the predominant respiratory quinone. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified phospholipids and one unidentified aminophospholipid. The major fatty acids were anteiso-C, C and iso-C. Physiological and biochemical features differentiated strain SCIV0701 from the closely related species. Based on the results of polyphasic taxonomic analysis, strain SCIV0701 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SCIV0701 (=GDMCC 1.2482=JCM 34672).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): agar-hydrolytic activity , Paenibacillus , polyphasic taxonomic analysis and rhizosphere
Funding
This study was supported by the:
  • Project by the Department of Science and Technology of Guangdong Province (Award 2019QN01N107)
    • Principle Award Recipient: qingpingwu
  • Key Laboratory of Guangdong Province (Award 2020B121201009)
    • Principle Award Recipient: qingpingwu
  • Guangzhou Science and Technology Program (Award 202201010461)
    • Principle Award Recipient: XinqiangXie
  • National Natural Science Foundation of China (Award 32202380)
    • Principle Award Recipient: HuiZhao
  • National Key R&D Program of China (Award 2021YFA0910200)
    • Principle Award Recipient: XinqiangXie
© 2023 The Authors
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2023-04-05
2025-05-14
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Paenibacillus soyae sp. nov., isolated from the rhizosphere of soya bean
Int J Syst Evol Microbiol 73, 005761 (2023); https://doi.org/10.1099/ijsem.0.005761
/content/journal/ijsem/10.1099/ijsem.0.005761
/content/journal/ijsem/10.1099/ijsem.0.005761
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