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Volume 73, Issue 2

sp. nov., a tetrachloroethene-respiring bacterium isolated from contaminated soil No Access

Abstract

Two anaerobic, tetrachloroethene- (PCE-) respiring bacterial isolates, designated strain ACS and strain ACS, were characterized using a polyphasic approach. Cells were Gram-stain-negative, motile, non-spore-forming and shared a vibrioid- to spirillum-shaped morphology. Optimum growth occurred at 30 °C and 0.1–0.4 % salinity. The pH range for growth was pH 5.5–7.5, with an optimum at pH 7.2. Hydrogen, formate, pyruvate and lactate as electron donors supported respiratory reductive dechlorination of PCE to -1,2-dichloroethene (DCE) in strain ACS and of PCE to trichloroethene (TCE) in strain ACS. Both strains were able to grow with pyruvate under microaerobic conditions. Nitrate, elemental sulphur, and thiosulphate were alternative electron acceptors. Autotrophic growth was not observed and acetate served as carbon source for both strains. The major cellular fatty acids were C 7, C, C and C 7. Both genomes feature a circular plasmid. Strains ACS and ACS were previously assigned to the candidate species 'Sulfurospirillum acididehalogenans'. Here, based on key genomic features and pairwise comparisons of whole-genome sequences, including average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity, strains ACS and ACS, '. Sulfurospirillum diekertiae' strains SL2-1 and SL2-2, and the unclassified sp. strain SPD-1 are grouped into one distinct species separate from previously described species. Compared to and , which dechlorinate PCE to DCE without substantial TCE accumulation, these five strains produce TCE or DCE as the end product. In addition, some cellular fatty acids (e.g., C 3OH, C iso 3OH, C 2OH) were detected in strains ACS and ACS but not in other species. On the basis of phylogenetic, physiological and phenotypic characteristics, . Sulfurospirillum acididehalogenans' and . Sulfurospirillum diekertiae' are proposed to be merged into one novel species within the genus , for which the name sp. nov. is proposed. The type strain is ACS (=JCM 33349= KCTC 15819=CGMCC 1.5292).

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  • Accepted:
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Keyword(s): anaerobic bacteria , organohalide respiration , reductive dechlorination , Sulfurospirillum and tetrachloroethene
Funding
This study was supported by the:
  • Key Research Program of Frontier Sciences, Chinese Academy of Sciences (Award ZDBS-LY-DQC038)
    • Principle Award Recipient: YiYang
  • Natural Science Foundation of China (Award 41907287)
    • Principle Award Recipient: YiYang
  • Natural Science Foundation of China (Award 41907220)
    • Principle Award Recipient: YiYang
  • Natural Science Foundation of China (Award 41977295)
    • Principle Award Recipient: JunYan
© 2023 The Authors
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2023-02-03
2024-04-19
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Sulfurospirillum diekertiae sp. nov., a tetrachloroethene-respiring bacterium isolated from contaminated soil
Int J Syst Evol Microbiol 73, 005693 (2023); https://doi.org/10.1099/ijsem.0.005693
/content/journal/ijsem/10.1099/ijsem.0.005693
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