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Volume 73, Issue 1

sp. nov., a new species isolated from the hot water distribution systems of a hospital and a shopping center during routine sampling No Access

Abstract

Two -like strains isolated from hot water distribution systems in 2012 have been characterized phenotypically, biochemically and genomically in terms of DNA relatedness. Both strains, HCPI-6 and EUR-108, exhibited biochemical phenotypic profiles typical of species. Cells were Gram-negative motile rods which grew on BCYEα agar but not on blood agar and displayed phenotypic characteristics typical of the family , including a requirement for -cysteine and testing catalase positive. Both strains were negative for oxidase, urease, nitrate reduction and hippurate negative, and non-fermentative. The major ubiquinone was Q12 (59.4 % HCPI-6) and the dominant fatty acids were C ω7 (28.4 % HCPI-6, ≈16 % EUR-108), C iso (≈22.5 % and ≈13 %) and C anteiso (19.5 % and ≈23.5 %, respectively). The percent G+C content of genomic DNA was determined to be 39.3 mol %. The 16S rRNA gene, sequence and comparative genome sequence-based analyses (average nucleotide identity, ANI; digital DNA–DNA hybridization, dDDH; and phylogenomic treeing) demonstrated that the strains represent a new species of the genus . The analysis based on the 16S rRNA gene sequences showed that the sequence similarities for both strains ranged from 98.8–90.1 % to other members of the genus. The core genome-based phylogenomic tree (protein-concatemer tree based on concatenation of 418 proteins present in single copy) revealed that these two strains clearly form a separate cluster within the genus . ANI and dDDH values confirmed the distinctiveness of the strains. Based on the genomic, genotypic and phenotypic findings from a polyphasic study, the isolates are considered to represent a single novel species, for which the name sp. nov. is proposed. The type strain is HCPI-6 (=CCUG 75071=CECT 30569).

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  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , average nucleotide identity , digital DNA–DNA hybridization , genome comparison , hot water , Legionella maioricensis , mip , novel species , phylogenomics and polyphasic taxonomy
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2023-01-17
2024-04-25
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Legionella maioricensis sp. nov., a new species isolated from the hot water distribution systems of a hospital and a shopping center during routine sampling
Int J Syst Evol Microbiol 73, 005686 (2023); https://doi.org/10.1099/ijsem.0.005686
/content/journal/ijsem/10.1099/ijsem.0.005686
/content/journal/ijsem/10.1099/ijsem.0.005686
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