Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 1

Morphological and genetic analysis of Zhao . 2015 and sp. nov. (Myxozoa: Ceratomyxidae) from the East China Sea No Access

Abstract

We describe Zhao . 2015 and sp. nov. (Myxozoa: Ceratomyxidae) from the East China Sea. was found in the gallbladders of 3/13 specimens of its type host, Temminck and Schlegel 1846 (Aulopiformes). Myxospore characters were consistent with the original description to which we have added small subunit (SSU) rRNA gene data. sp. nov. was found in gallbladders of 3/13 specimens of . and 5/13 specimens of Jordan and Snyder 1901 (Ophidiiformes). Mature myxospores of sp. nov. were crescentic in sutural view, with a deeply concave posterior angle 142.2±8.2° (125.8‒158.2°) and an arched anterior side. Shell valves were smooth and equal, 20.9±1.9 (17.3‒24.7) µm thick and 9.2±0.5 (8.1‒9.9) µm long, and joined at a straight, thin sutural plane passing between two nematocysts (polar capsules). The nematocysts were equal-sized, pyriform, 2.6±0.2 (2.4‒2.9) µm long and 2.7±0.2 (2.4‒3.3) µm wide, with their tapered ends pointed toward each other, located in the anterior third of the spore. Sequences of the SSU rRNA gene and internal transcribed spacer 1 showed that the isolates of sp. nov. obtained from and were identical. The SSU rRNA gene sequence of sp. nov. was distinct from all known myxosporeans and clustered with , and then with Kalatzis, Kokkari and Katharios 2013, both of which also infect Aulopiformes fishes.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ITS1 , myxospore , Neobythites sivicola , Saurida elongata and SSU rRNA gene
Funding
This study was supported by the:
  • Science and Technology Research Program of Chongqing Municipal Education Commission (Award KJQN201900505)
    • Principle Award Recipient: ChengzhongYang
  • Venture and Innovation Support Program for Chongqing Overseas Returnees (Award cx2018108)
    • Principle Award Recipient: ChengzhongYang
  • National Natural Science Foundation of China (Award 32070432)
    • Principle Award Recipient: NotApplicable
  • National Natural Science Foundation of China (Award 31970409)
    • Principle Award Recipient: YuanjunZhao
© 2023 The Authors
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.005662
2023-01-04
2025-04-23
Loading full text...

Full text loading...

References

  1. Whipps CM, Kent ML. Phylogeography of the cosmopolitan marine parasite Kudoa thyrsites (Myxozoa: Myxosporea). J Eukaryot Microbiol 2006; 53:364–373 [View Article] [PubMed]
     [Google Scholar]
  2. Lom J, Dyková I. Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasit 2006; 53:1–36 [View Article]
     [Google Scholar]
  3. Özer A, Yurakhno V, Öztürk T, Kornyychuk YM. Myxosporean parasites of Ceratomyxa merlangi and Myxidium gadi in whiting Merlangius merlangus: a comparative epizootiological analysis based on samples from two localities off southern and northern coasts of the Black Sea. Parasitol Res 2017; 116:2463–2469 [View Article] [PubMed]
     [Google Scholar]
  4. Skuratovskaya EN, Yurakhno VM, Zavyalov AV. The influence of parasitic infection on the Black Sea Whiting, Merlangius merlangus euxinus (Gadidae), morphophysiological and biochemical parameters. Vestnik Zoologii 2013; 47:309–317 [View Article]
     [Google Scholar]
  5. Atkinson SD, Hallett SL, Bartholomew JL. Genotyping of individual Ceratonova shasta (Cnidaria: Myxosporea) myxospores reveals intra-spore ITS-1 variation and invalidates the distinction of genotypes II and III. Parasitology 2018; 145:1588–1593 [View Article] [PubMed]
     [Google Scholar]
  6. Breyta R, Atkinson SD, Bartholomew JL. Evolutionary dynamics of Ceratonova species (Cnidaria: Myxozoa) reveal different host adaptation strategies. Infect Genet Evol 2020; 78:104081 [View Article] [PubMed]
     [Google Scholar]
  7. Chen W, Yang C, Whipps CM, Peng Z, Zhao Y. Taxonomy on three novel species of Sphaeromyxa Thélohan 1892 (Myxozoa, Bivalvulida, Sphaeromyxidae) with insight into the evolution of the genus. Parasitol Res 2020; 119:1493–1503 [View Article] [PubMed]
     [Google Scholar]
  8. Qing X, Chen W, Li C, Yang C, Zhao Y. The first report of two Sphaeromyxa species (Myxozoa: Bivalvulida) from the South China Sea: Sphaeromyxa scorpaena n. sp. from long-fingered scorpionfish (Scorpaenodes albaiensis) and Sphaeromyxa theraponi from tiger perch (Terapon jarbua). Parasitol Res 2020; 119:3617–3625 [View Article]
     [Google Scholar]
  9. Heiniger H, Adlard RD. Molecular identification of cryptic species of Ceratomyxa Thélohan, 1892 (Myxosporea: Bivalvulida) including the description of eight novel species from apogonid fishes (Perciformes: Apogonidae) from Australian waters. Acta Parasitol 2013; 58:342–360 [View Article] [PubMed]
     [Google Scholar]
  10. Garbouj M, Rangel LF, Santos MJ, Bahri S. Ceratomyxa gouletti n. sp. (Myxosporea: Ceratomyxidae), a parasite of the red scorpionfish Scorpaena scrofa (L.) from Tunisian waters. Parasitol Res 2018; 117:1933–1939 [View Article] [PubMed]
     [Google Scholar]
  11. Eiras JC. Synopsis of the species of Ceratomyxa Thélohan, 1892 (Myxozoa: Myxosporea: Ceratomyxidae). Syst Parasitol 2006; 65:49–71 [View Article] [PubMed]
     [Google Scholar]
  12. Eiras JC, Cruz C, Saraiva A. Synopsis of the species of Ceratomyxa Thélohan, 1892 (Cnidaria, Myxosporea, Ceratomyxidae) described between 2007 and 2017. Syst Parasitol 2018; 95:427–446 [View Article] [PubMed]
     [Google Scholar]
  13. Gunter N, Adlard R. The demise of Leptotheca Thélohan, 1895 (Myxozoa: Myxosporea: Ceratomyxidae) and assignment of its species to Ceratomyxa Thélohan, 1892 (Myxosporea: Ceratomyxidae), Ellipsomyxa Køie, 2003 (Myxosporea: Ceratomyxidae), Myxobolus Bütschli, 1882 and Sphaerospora Thélohan, 1892 (Myxosporea: Sphaerosporidae). Syst Parasitol 2010; 75:81–104 [View Article]
     [Google Scholar]
  14. Sanil NK, Chandran A, Shamal P, Binesh CP. Molecular and morphological descriptions of Ceratomyxa collarae n. sp. and Ceratomyxa leucosternoni n. sp. from marine ornamental fishes of Indian waters. Parasitol Res 2017; 116:529–537 [View Article]
     [Google Scholar]
  15. Zhang D, Zhao Y, Yang S, Yang C. Morphological and molecular identification of a novel species, Ceratomyxa siganicola n. sp. (Myxozoa: Ceratomyxidae) from Siganus fuscescens, in East China Sea. Acta Parasitol 2019; 64:596–602 [View Article]
     [Google Scholar]
  16. Qiao Y, Shao Y, Pengsakul T, Chen C, Zheng S et al. Morphological and molecular characterization of Ceratomyxa batam n. sp. (Myxozoa: Ceratomyxidae) infecting the gallbladder of the cultured Trachinotus ovatus (Perciformes: Carangidae) in Batam Island, Indonesia. Parasitol Res 2019; 118:1647–1651 [View Article]
     [Google Scholar]
  17. Bouderbala K, Rangel LF, Santos MJ. Ceratomyxa mennani n. sp. (Myxosporea: Bivalvulida) parasitizing the gallbladder of the dusky grouper Epinephelus marginatus (Serranidae) from Tunisian waters. Parasitol Res 2020; 119:1515–1522 [View Article]
     [Google Scholar]
  18. Zhao Y, Zhou Y, Kent ML, Whipps CM. Replacement of the preoccupied name Davisia Laird 1953 and description of a new myxozoan species (Myxosporea: Sinuolineidae) from Sebastiscus marmoratus (Cuvier, 1829) in the East China Sea. J Parasitol 2008; 94:269–279 [View Article]
     [Google Scholar]
  19. Zhao Y, Al-Farraj SA, AL-Rasheid KAS, Song W. Data on ten new myxosporean parasites (Myxozoa, Myxosporea, Bivalvulida) from the Yellow Sea, China. Acta Protozool 2015; 54:305–323 [View Article]
     [Google Scholar]
  20. Yang C, Zhou Y, Zhao Y, Huang W, Huang C. Erection of Unicapsulocaudum mugilum gen. et sp. nov. (Myxozoa: Ceratomyxidae) based on its morphological and molecular data. J Nat Hist 2017; 51:457–467 [View Article]
     [Google Scholar]
  21. Huang Y, Zhao Y, Zhou Y, Yang C. First molecular evidence of Ceratomyxa epinephela (Myxozoa: Ceratomyxidae) and its genetic variation from different host species. Acta Hydrobiol Sin 2020; 44:1263–1269 [View Article]
     [Google Scholar]
  22. Zhao Y, Ma C, Song W. Illustrated guide to the identification of pathogenetic protozoa in maricuture. II diagnostic methods for the myxosporean. J Ocean Univ Qingdao 2001; 31:681–688
     [Google Scholar]
  23. Barta JR, Martin DS, Liberator PA, Dashkevicz M, Anderson JW et al. Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences. J Parasitol 1997; 83:262–271 [View Article] [PubMed]
     [Google Scholar]
  24. Wang S, Zhao Y, Du Y, Tang F. Morphological redescription and molecular identification of Trichodina reticulata Hirschmann & Partsch, 1955 (Ciliophora, Mobilida, Trichodinidae) with the supplemental new data of SSU rDNA and ITS-5.8S rDNA. J Eukaryot Microbiol 2019; 66:447–459 [View Article]
     [Google Scholar]
  25. Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PDN. DNA barcoding Australia’s fish species. Philos Trans R Soc Lond B Biol Sci 2005; 360:1847–1857 [View Article] [PubMed]
     [Google Scholar]
  26. Gunter NL, Adlard RD. Seven new species of Ceratomyxa Thélohan, 1892 (Myxozoa) from the gall-bladders of serranid fishes from the Great Barrier Reef, Australia. Syst Parasitol 2009; 73:1–11 [View Article] [PubMed]
     [Google Scholar]
  27. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article] [PubMed]
     [Google Scholar]
  28. Castresana J. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 2000; 17:540–552 [View Article] [PubMed]
     [Google Scholar]
  29. Ronquist F, Huelsenbeck JP. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 2003; 19:1572–1574 [View Article] [PubMed]
     [Google Scholar]
  30. Posada DL, Crandall KA. MODELTEST: testing the model of DNA substitution. Bioinformatics 1998; 14:817–818 [View Article] [PubMed]
     [Google Scholar]
  31. Rambaut A, Drummond A. Tracer V1.3: MCMC Trace Analysis Tool. Institute of Evolutionary Biology, University of Edinburgh 2005 http://tree.bio.ed.ac.uk/software/tracer/
     [Google Scholar]
  32. Stamatakis A, Hoover P, Rougemont J. A rapid bootstrap algorithm for the RAxML Web servers. Syst Biol 2008; 57:758–771 [View Article] [PubMed]
     [Google Scholar]
  33. Rice P, Longden I, Bleasby A. EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet 2000; 16:276–277 [View Article] [PubMed]
     [Google Scholar]
  34. Moser M, Love MS, Jensen LA. Myxosporida (Protozoa) in California rockfish, Sebastes spp. J Parasitol 1976; 62:690–692 [PubMed]
     [Google Scholar]
  35. Wierzbicka J. Ceratomyxa americana n. sp. (Myxospora,Bivalvulida), a parasite of mackerel (Scomber scombrus L.). Acta Ichthyol Piscat 1987; 17:93–96
     [Google Scholar]
  36. Gunter NL, Whipps CM, Adlard RD. Ceratomyxa (Myxozoa: Bivalvulida): robust taxon or genus of convenience?. Int J Parasitol 2009; 39:1395–1405 [View Article] [PubMed]
     [Google Scholar]
  37. Noble ER. Seasonal variations in hos–parasite relations between fish and their protozoa. J Mar Biol Ass 1957; 36:143–155 [View Article]
     [Google Scholar]
  38. Atkinson SD, Bartholomew JL. Disparate infection patterns of Ceratomyxa shasta (Myxozoa) in rainbow trout Oncorhynchus mykiss and Chinook salmon Oncorhynchus tshawytscha correlate with internal transcribed spacer-1 sequence variation in the parasite. Int J Parasitol 2010; 40:599–604 [View Article]
     [Google Scholar]
  39. Gunter NL, Adlard RD. Bivalvulidan (Myxozoa: Myxosporea) parasites of damselfishes with description of twelve novel species from Australia’s Great Barrier Reef. Parasitology 2008; 135:1165–1178 [View Article] [PubMed]
     [Google Scholar]
  40. Capodifoglio KR, Adriano EA, Milanin T, Silva MR, Maia AA. Morphological, ultrastructural and phylogenetic analyses of Myxobolus hilarii n. sp. (Myxozoa, Myxosporea), a renal parasite of farmed Brycon hilarii in Brazil. Parasitol Int 2016; 65:184–190 [View Article]
     [Google Scholar]
  41. Zhang B, Zhai Y, Liu Y, Gu Z. Myxobolus pseudowulii sp. n. (Myxozoa: Myxosporea), a new skin parasite of yellow catfish Tachysurus fulvidraco (Richardson) and redescription of Myxobolus voremkhai (Akhmerov, 1960). Folia Parasit 2017; 64:e030 [View Article]
     [Google Scholar]
  42. Vieira D, Tagliavini VP, Abdallah VD, Azevedo RK. Myxobolus imparfinis n. sp. (Myxozoa: Myxosporea), a new gill parasite of Imparfinis mirini Haseman (Siluriformes: Heptapteridae) in Brazil. Syst Parasitol 2018; 95:309–318 [View Article]
     [Google Scholar]
  43. Gao L, Zhang J, Yang C, Zhao Y. Myxobolus jialingensis n. sp. (Myxozoa: Myxobolidae) infecting urinary bladder and hepatopancreas of yellowhead catfish Tachysurus fulvidraco from China. Zootaxa 2020; 4819:179–186 [View Article]
     [Google Scholar]
  44. Fiala I, Hlavničková M, Kodádková A, Freeman MA, Bartošová-Sojková P et al. Evolutionary origin of Ceratonova shasta and phylogeny of the marine myxosporean lineage. Mol Phylogenet Evol 2015; 86:75–89 [View Article]
     [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.005662
Loading
Morphological and genetic analysis of Ceratomyxa saurida Zhao et al. 2015 and Ceratomyxa mai sp. nov. (Myxozoa: Ceratomyxidae) from the East China Sea
Int J Syst Evol Microbiol 73, 005662 (2023); https://doi.org/10.1099/ijsem.0.005662
/content/journal/ijsem/10.1099/ijsem.0.005662
/content/journal/ijsem/10.1099/ijsem.0.005662
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error