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Volume 72, Issue 11

sp. nov., isolated from the rhizosphere, and reclassification of as comb. nov. No Access

Abstract

A Gram-stain-positive, facultative anaerobic endospore-forming bacterium, which originated from roots/rhizosphere of maize (), was investigated for its taxonomic position. On the basis of 16S rRNA gene sequence similarities, strain JJ-3 was grouped together with species showing the highest similarities to (98.8 %) and the three species , and (all 98.6 %). The 16S rRNA gene sequence similarities to the sequences of the type strains of other species were lower than 98.5 %. The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between the JJ-3 genome assembly and those of the other type strains were <83, <85 and <27 %, respectively. Chemotaxonomic features supported the grouping of the strain to the genus e.g. the major fatty acids were C anteiso and C iso, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and the major quinone was menaquinone MK-7. Physiological and biochemical test results were slightly different from those of the most closely related species. For this reason, JJ-3 represents a novel species of the genus , for which we propose the name sp. nov., with JJ-3 (= CIP 111895=LMG 32087=DSM 111784=CCM 9084) as the type strain. We also propose to reclassify as comb. nov. based mainly on the results of phylogenomic and conserved signature indel analyses.

  • Received:
  • Accepted:
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Keyword(s): Bacillaceae , Neobacillus , new species , polyphasic taxonomy and root isolate
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2022-11-03
2024-04-19
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Neobacillus rhizosphaerae sp. nov., isolated from the rhizosphere, and reclassification of Bacillus dielmonensis as Neobacillus dielmonensis comb. nov.
Int J Syst Evol Microbiol 72, 005636 (2022); https://doi.org/10.1099/ijsem.0.005636
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