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Volume 72, Issue 12

sp. nov., an actinobacterium isolated from peat swamp forest soil No Access

Abstract

An actinobacterium strain PLK6-54 was isolated from Lankwai peat swamp forest soil collected from Yala province, Thailand. Strain PLK6-54 exhibited morphological and chemotaxonomic properties described for the genus . It formed a spiral spore chain directly on aerial mycelium. Growth was observed between 20 and 40 °C and at pH 5–8. The maximum NaCl for growth was 2 % (w/v). -Diaminopimelic acid, arabinose and ribose were detected in the whole-cell hydrolysate. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside were detected as the phospholipids. The major menaquinones were MK-10(H) and MK-9(H). The major cellular fatty acids were iso-C, anteiso-C and iso-C. 16S rRNA gene sequence data supported the assignment of strain PLK6-54 to the genus and showed that NBRC 102073 (99.0 %) was the closest relative. Moreover, the average nucleotide identity- (85.5 %) and digital DNA–DNA hybridization (30.7 %) values reported between strain PLK6-54 and its closest neighbour were below the threshold values for delineation of a novel species. Strain PLK6-54 could be distinguished from related validly described species by several phenotypic properties. The combination of genotypic and phenotypic data indicated that strain PLK6-54 is representative of a novel species of the genus . The name sp. nov. is proposed for strain PLK6-54. The type strain is PLK6-54 (=TBRC 11250=NBRC 114297).

  • Received:
  • Accepted:
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Keyword(s): 16S rRNA gene , actinomycete , peat swamp forest , Streptomyces and whole-genome analysis
Funding
This study was supported by the:
  • School of Science, King Mongkut’s Institute of Technology Ladkrabang (Award 2563-02-05-29)
    • Principle Award Recipient: ChittiThawai
© 2022 The Authors
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Streptomyces acidipaludis sp. nov., an actinobacterium isolated from peat swamp forest soil
Int J Syst Evol Microbiol 72, 005626 (2023); https://doi.org/10.1099/ijsem.0.005626
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