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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 72, Issue 11

sp. nov. isolated from a tidal flat of the Yellow Sea No Access

Abstract

A novel species of the genus , designated ASW11-100, was isolated from a tidal flat sediment in the Yellow Sea, PR China. Phylogenetic analysis based on 16S rRNA gene sequences and single-copy orthologous clusters revealed that strain ASW11-100 belonged to the genus , and exhibited 16S rRNA gene sequence similarities of 98.9, 98.8 and 98.7 % to HSMS-1, GHTF-27 and KT0803, respectively. The genome of strain ASW11-100 harbours 2950 protein-coding genes and 105 carbohydrate-active enzymes including 38 glycoside hydrolases. Seventeen of the glycoside hydrolases are organized in five distinct polysaccharide utilization loci, which are predicted to involve in the degradation of starch, glucans, arabinoxylans, arabinomannan, arabinans and arabinogalactans. The genomic DNA G+C content was 37.3 mol%. The digital DNA–DNA hybridization and average nucleotide identity values between strain ASW11-100 and its closely related relatives were in ranges of 19.8–23.9% and 76.6–80.9 %, respectively. Cells of the isolate were Gram-negative, aerobic, non-flagellated and short rod-shaped. Carotenoid pigments were produced, but flexirubin-type pigments were absent. The major fatty acids (>10 %) were iso-C, iso-C 3-OH and summed feature 3 (C 6 and/or C 7). The sole respiratory quinone was menaquinone-6 and the major polar lipid was phosphatidylethanolamine. Based on the above polyphasic evidence, strain ASW11-100 should be considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is ASW11-100 (=KCTC 82502=MCCC 1K05580).

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  • Accepted:
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Keyword(s): Gramella sediminis , polyphasic taxonomy and sediment
Funding
This study was supported by the:
  • Science and Technology Innovation Fund Project of Shanxi Agricultural University (Award 2020BQ07)
    • Principle Award Recipient: Cheng-QiangXia
  • Excellent Talents Come to Shanxi to Reward Scientific Research Projects (Award SXYBKY2019025)
    • Principle Award Recipient: YiLi
  • Excellent Talents Come to Shanxi to Reward Scientific Research Projects (Award SXYBKY2019024)
    • Principle Award Recipient: Cheng-QiangXia
  • National Natural Science Foundation of China (Award 31972590)
    • Principle Award Recipient: Cai-XiaPei
  • National Natural Science Foundation of China (Award 32002143)
    • Principle Award Recipient: Cheng-QiangXia
  • Youth Foundation of the Shanxi Science and Technology Department (Award 201901D211375)
    • Principle Award Recipient: Cheng-QiangXia
© 2022 The Authors
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2022-11-09
2025-03-21
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Gramella sediminis sp. nov., isolated from a tidal flat of the Yellow Sea
Int J Syst Evol Microbiol 72, 005621 (2022); https://doi.org/10.1099/ijsem.0.005621
/content/journal/ijsem/10.1099/ijsem.0.005621
/content/journal/ijsem/10.1099/ijsem.0.005621
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