1887

Abstract

A novel, Gram-stain-negative, rod-shaped, strictly anaerobic bacterium of genus of the phylum Bacteroidota, named strain JNU-WLY501, was isolated from pit clay used to produce strong aroma-type liquor in PR China. The genomic DNA G+C content and genome size of JNU-WLY501 were 41.4 % and 3.9 Mbp, respectively. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that JNU-WLY501 was closely related to DSM 18083 (95.7 %) and M3/6 (94.9 %). The pairwise average nucleotide identity based on and average amino acid identity values of JNU-WLY501 compared with M3/6 were 73.6 and 77.3 %, respectively, which both were lower than the threshold values for bacterial species delineation. The strain grew at 20–40 °C, with optimum growth at 37 °C. The pH range for growth was 5.4–9.1, with optimum growth at pH 7.5. The sodium chloride range for growth was 0.0–4.0 %, with optimum growth at 0 %. The strain did not use glucose, maltose, fructose or starch. Yeast extract, tryptone and peptone supported the growth of JNU-WLY501, and the main fermentation products were acetate and propionate. The predominant cellular fatty acids (>5 %) of JNU-WLY501 were anteiso-C (30.6 %), anteiso-C (26.1 %), C (7.7 %), iso-C (5.0 %) and iso-C (5.0 %). The respiratory quinone of JNU-WLY501 was MK-5. On the basis of the morphological, physiological, biochemical, chemotaxonomic, genotypic and phylogenetic results, JNU-WLY501 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JNU-WLY501 (=GDMCC 1.2686=JCM 34753).

Funding
This study was supported by the:
  • Chinese Baijiu Industrial Technology Innovation Strategic Alliance project (Award 2018-3)
    • Principle Award Recipient: YanXu
  • National Natural Science Foundation of China (Award 32172177)
    • Principle Award Recipient: CongRen
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/content/journal/ijsem/10.1099/ijsem.0.005612
2022-11-24
2024-05-26
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References

  1. Fu J, Chen L, Yang S, Li Y, Jin L et al. Metagenome and analysis of metabolic potential of the microbial community in pit mud used for Chinese strong-flavor liquor production. Food Res Int 2021; 143:110294 [View Article]
    [Google Scholar]
  2. Hu XL, Du H, Ren C, Xu Y. Illuminating anaerobic microbial community and cooccurrence patterns across a quality gradient in Chinese liquor fermentation pit muds. Appl Environ Microbiol 2016; 82:2506–2515 [View Article]
    [Google Scholar]
  3. Chai L-J, Qian W, Zhong X-Z, Zhang X-J, Lu Z-M et al. Mining the factors driving the evolution of the pit mud microbiome under the impact of long-term production of strong-flavor baijiu. Appl Environ Microbiol 2021; 87:e0088521 AEM0088521 [View Article]
    [Google Scholar]
  4. Gao J, Liu G, Li A, Liang C, Ren C et al. Domination of pit mud microbes in the formation of diverse flavour compounds during Chinese strong aroma-type baijiu fermentation. LWT 2021; 137:110442 [View Article]
    [Google Scholar]
  5. Wang Q, Liu K, Liu L, Zheng J, Chen T et al. Correlation analysis between aroma components and microbial communities in Wuliangye-flavor raw liquor based on HS-SPME/LLME-GC–MS and PLFA. Food Res Int 2021; 140:109995 [View Article]
    [Google Scholar]
  6. Fan WL, Qian MC. Characterization of aroma compounds of chinese “Wuliangye” and “Jiannanchun” liquors by aroma extract dilution analysis. J Agric Food Chem 2006; 54:2695–2704 [View Article] [PubMed]
    [Google Scholar]
  7. Wang X, Du H, Xu Y. Source tracking of prokaryotic communities in fermented grain of Chinese strong-flavor liquor. Int J Food Microbiol 2017; 244:27–35 [View Article] [PubMed]
    [Google Scholar]
  8. Zhang H, Meng Y, Wang Y, Zhou Q, Li A et al. Prokaryotic communities in multidimensional bottom-pit-mud from old and young pits used for the production of Chinese Strong-Flavor Baijiu. Food Chem 2020; 312:126084 [View Article]
    [Google Scholar]
  9. Gu Y, Zhu X, Lin F, Shen C, Li Y et al. Caproicibacterium amylolyticum gen. nov., sp. nov., a novel member of the family Oscillospiraceae isolated from pit clay used for making Chinese strong aroma-type liquor. Int J Syst Evol Microbiol 2021; 71: [View Article]
    [Google Scholar]
  10. Wang H, Gu Y, Zhao D, Qiao Z, Zheng J et al. Caproicibacterium lactatifermentans sp. nov., isolated from pit clay used for the production of Chinese strong aroma-type liquor. Int J Syst Evol Microbiol 2022; 72: [View Article] [PubMed]
    [Google Scholar]
  11. Tao Y, Li J, Rui J, Xu Z, Zhou Y et al. Prokaryotic communities in pit mud from different-aged cellars used for the production of Chinese strong-flavored liquor. Appl Environ Microbiol 2014; 80:2254–2260 [View Article] [PubMed]
    [Google Scholar]
  12. Lu M, Zhou W, Ji F, Wu J, Nie Y et al. Profiling prokaryotic community in pit mud of Chinese strong-aroma type liquor by using oligotrophic culturing. Int J Food Microbiol 2021; 337:108951 [View Article] [PubMed]
    [Google Scholar]
  13. Holdeman LV, Cato EP, Moore WEC. Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University; 1977
    [Google Scholar]
  14. Lányi B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1988; 19:1–67 [View Article]
    [Google Scholar]
  15. de Lillo A, Ashley FP, Palmer RM, Munson MA, Kyriacou L et al. Novel subgingival bacterial phylotypes detected using multiple universal polymerase chain reaction primer sets. Oral Microbiol Immunol 2006; 21:61–68 [View Article] [PubMed]
    [Google Scholar]
  16. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549 [View Article]
    [Google Scholar]
  17. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article]
    [Google Scholar]
  18. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  19. Emms DM, Kelly S. OrthoFinder: solving fundamental biases in whole genome comparisons dramatically improves orthogroup inference accuracy. Genome Biol 2015; 16:157 [View Article]
    [Google Scholar]
  20. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 2013; 30:772–780 [View Article]
    [Google Scholar]
  21. Capella-Gutiérrez S, Silla-Martínez JM, Gabaldón T. trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 2009; 25:1972–1973 [View Article]
    [Google Scholar]
  22. Jones DT, Taylor WR, Thornton JM. The rapid generation of mutation data matrices from protein sequences. Bioinformatics 1992; 8:275–282 [View Article]
    [Google Scholar]
  23. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article]
    [Google Scholar]
  24. Richter M, Rosselló-Móra R, Oliver Glöckner F, Peplies J. JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics 2016; 32:929–931 [View Article]
    [Google Scholar]
  25. Darling ACE, Mau B, Blattner FR, Perna NT. Mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res 2004; 147:1394–1403 [View Article]
    [Google Scholar]
  26. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci 2009; 106:19126–19131 [View Article]
    [Google Scholar]
  27. Nicholson AC, Gulvik CA, Whitney AM, Humrighouse BW, Bell ME et al. Division of the genus Chryseobacterium: observation of discontinuities in amino acid identity values, a possible consequence of major extinction events, guides transfer of nine species to the genus Epilithonimonas, eleven species to the genus Kaistella, and three species to the genus Halpernia gen. nov., with description of Kaistella daneshvariae sp. nov. and Epilithonimonas vandammei sp. nov. derived from clinical specimens. Int J Syst Evol Microbiol 2020; 70:4432–4450 [View Article]
    [Google Scholar]
  28. Qin Q-L, Xie B-B, Zhang X-Y, Chen X-L, Zhou B-C et al. A proposed genus boundary for the prokaryotes based on genomic insights. J Bacteriol 2014; 196:2210–2215 [View Article] [PubMed]
    [Google Scholar]
  29. Negrete-Abascal E, Reyes ME, García RM, Vaca S, Girón JA et al. Flagella and motility in Actinobacillus pleuropneumoniae. J Bacteriol 2003; 185:664–668 [View Article] [PubMed]
    [Google Scholar]
  30. Athalye M, Noble WC, Minnikin DE. Analysis of cellular fatty acids by gas chromatography as a tool in the identification of medically important coryneform bacteria. J Appl Bacteriol 1985; 58:507–512 [View Article] [PubMed]
    [Google Scholar]
  31. Minnikin DE, Collins MD, Goodfellow M. Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 1979; 47:87–95 [View Article]
    [Google Scholar]
  32. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article] [PubMed]
    [Google Scholar]
  33. Collins MD, McCarthy AJ, Cross T. New highly saturated members of the vitamin K2 series from Thermomonospora. Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I Abt Originale C: Allgemeine, angewandte und ökologische Mikrobiologie 1979; 3:358–363 [View Article]
    [Google Scholar]
  34. Collins MD, Shah HN, Minnikin DE. A note on the separation of natural mixtures of bacterial menaquinones using reverse phase thin-layer chromatography. J Appl Bacteriol 1980; 48:277–282 [View Article]
    [Google Scholar]
  35. Hahnke S, Langer T, Koeck DE, Klocke M. Description of Proteiniphilum saccharofermentans sp. nov., Petrimonas mucosa sp. nov. and Fermentimonas caenicola gen. nov., sp. nov., isolated from mesophilic laboratory-scale biogas reactors, and emended description of the genus Proteiniphilum. Int J Syst Evol Microbiol 2016; 66:1466–1475 [View Article]
    [Google Scholar]
  36. Chen S, Dong X. Proteiniphilum acetatigenes gen. nov., sp. nov., from a UASB reactor treating brewery wastewater. Int J Syst Evol Microbiol 2005; 55:2257–2261 [View Article]
    [Google Scholar]
  37. Grabowski A, Tindall BJ, Bardin V, Blanchet D, Jeanthon C. Petrimonas sulfuriphila gen. nov., sp. nov., a mesophilic fermentative bacterium isolated from a biodegraded oil reservoir. Int J Syst Evol Microbiol 2005; 55:1113–1121 [View Article] [PubMed]
    [Google Scholar]
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