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Volume 72, Issue 10

sp. nov., a novel salt-tolerant bacterium isolated from a saline lake sediment No Access

Abstract

A salt-tolerant bacterium, designated strain EGI L200015, was isolated from saline lake sediment in Xinjiang Uygur Autonomous Region, PR China. The taxonomic position of the isolate was determined using polyphasic taxonomic analysis and phylogenomic analysis. Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that EGI L200015 formed a distinct clade with KCTC 12718 with sequence identity of 98.3%. The novel isolate could be distinguished from species of the genus by its distinct phenotypic, physiological and genotypic characteristics. Cells of EGI L200015 were aerobic, Gram-stain-positive, non-motile and rod-shaped. Optimal growth conditions for EGI L200015 occurred on marine agar 2216 at pH 8.0 at 30 °C. The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C, iso-C, iso-C and anteiso-C. The detected polar lipids of included diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. On the basis of the genome sequence data, the DNA G+C content of EGI L200015 was 41.6 %. On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of the proposed novel isolate is EGI L200015 (= KCTC 43363 = CGMCC 1.19260).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): sediments , novel species , Pseudalkalibacillus , Pseudalkalibacillus salsuginis and Symphony Lake
Funding
This study was supported by the:
  • Xinjiang Uygur Autonomous Region regional coordinated innovation project (Award 2021E01018)
    • Principle Award Recipient: Wen-JunLi
  • Science and Technology Department of Xinjiang Uygur Autonomous Region (Award 2020E01047)
    • Principle Award Recipient: Wen-JunLi
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2019FY100701)
    • Principle Award Recipient: Bao-ZhuFang
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: Bao-ZhuFang
© 2022 Sun Yat-Sen University
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2022-10-25
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Pseudalkalibacillus salsuginis sp. nov., a novel salt-tolerant bacterium isolated from a saline lake sediment
Int J Syst Evol Microbiol 72, 005597 (2022); https://doi.org/10.1099/ijsem.0.005597
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