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Abstract

A Gram-stain-negative, facultatively anaerobic, flagellated and coccoid, ovoid or rod-shaped bacterial strain, NIFS-20-8, was isolated from the intestine of an olive flounder () from the East Sea, Republic of Korea. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain NIFS-20-8 fell within the clade comprising the type strains of species. Strain NIFS-20-8 exhibited 16S rRNA gene sequence similarities of 97.2 and 97.1 % to the type strains of and , respectively, and of 96.6–97.0 % to the type strains of the other species. The average nucleotide identity and digital DNA–DNA hybridization values between the genomic sequence of strain NIFS-20-8 and those of the type strains of four species were 73.8–75.0 and 19.8–21.1 %, respectively. The DNA G+C content of strain NIFS-20-8 from genomic sequence data was 50.55 mol%. Strain NIFS-20-8 contained Q-8 as the predominant ubiquinone and summed feature 3 (C 7 and/or C 6), C and C 7 as the major fatty acids. The major polar lipids detected in stain NIFS-20-8 were phosphatidylethanolamine and phosphatidylglycerol. Distinguishing phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain NIFS-20-8 is separated from recognized species. On the basis of the data presented here, strain NIFS-20-8 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NIFS-20-8 (= KCTC 82873=NBRC 115237).

Funding
This study was supported by the:
  • Rural Development Administration (Award PJ015247)
    • Principle Award Recipient: Jung-HoonYoon
  • Instituto Nacional de Pesca y Acuacultura (Award R2022029)
    • Principle Award Recipient: Jung-HoonYoon
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2022-12-02
2024-11-09
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