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Volume 72, Issue 10

sp. nov. isolated from roots No Access

Abstract

An aerobic, Gram-staining-positive, endospore-forming bacterium, isolated from the rhizosphere of roots of maize (), was taxonomically studied. On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125 clustered together with species of the genus and showed the highest similarities with (98.7 %). The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus were <98.4 %. The genome sequence of JJ-125 was 4 516 360 bp long and had a DNA G+C content of 37.3 %. A DNA–DNA hybridization with the type strain of DSM 23010 resulted in values of 42.3 and 43.9 % (reciprocal). The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between the JJ-125 genome assembly and those of the other type strains of species of the genus were <75%, <80 % and <21 %, respectively. Chemotaxonomic features supported the grouping of the strain with the genus e.g. the major fatty acids included iso-C iso-C ω10 and iso-C, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was -diaminopimelic acid. Physiological and biochemical test results were also different from those of the most closely related species. As a consequence, JJ-125 represents a novel species of the genus , for which we propose the name sp. nov., with JJ-125 (= CIP 111883 = LMG 32156 = CCM 9046) as the type strain.

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Keyword(s): firmicutes , novel species , polyphasic taxonomy , root isolate and Sutcliffiella
© 2022 The Authors
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2022-10-11
2024-04-25
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Sutcliffiella rhizosphaerae sp. nov. isolated from roots
Int J Syst Evol Microbiol 72, 005590 (2022); https://doi.org/10.1099/ijsem.0.005590
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