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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 72, Issue 11

sp. nov., isolated from the sediment on the surface of plastics and proposal of a novel genus gen. nov. based on the genome-based phylogeny of the family No Access

Abstract

A Gram-stain-negative, rod-shaped and orange-pigmented bacterial strain designated 10MBP4-2-1 was isolated from the sediment on the surface of a plastic straw collected from oyster-farming areas in Quanzhou Bay, PR China. Catalase activity and oxidase activity were positive. Flexirubin-type pigment was absent. The 16S rRNA gene of strain 10MBP4-2-1 showed highest sequence similarity to YM8-076 of 98.3 %. Phylogenetic analysis based on 16S rRNA gene sequences and 120 conserved concatenated proteins indicated that strain 10MBP4-2-1 was affiliated to the genus and formed a monophyletic clade with YM8-076. The digital DNA–DNA hybridization, average nucleotide identity and average amino acid identity values between strain 10MBP4-2-1 and YM8-076 were estimated to be 17.7, 70.4 and 70.3 %, respectively. The respiratory quinone was menaquinone 7. The major fatty acid composition was iso-C, iso-C 3-OH and summed feature 3 (C 7 and/or C 6). The draft genome size was 5 191 941 bp with DNA G+C content of 39.2 %. Based on phylogenetic analyses and whole genomic comparisons, strain 10MBP4-2-1 represents a novel species, for which the name sp. nov. is proposed. The type strain is 10MBP4-2-1 (=MCCC 1K07073=KCTC 92101). Additionally, phylogeny and whole genomic comparison of the family placed and the remaining (currently comprising 11 species) in two clearly distinguishable clades recognized at the genus level. Thus, a novel genus named gen. nov. is proposed to accommodate the 11 species. Our study provides a taxonomic framework for the family based on genomic data.

  • Received:
  • Accepted:
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Keyword(s): Lewinella , Neolewinella , phylogeny , polyphasic taxonomy and Portibacter
Funding
This study was supported by the:
  • Key Laboratory of Marine Biological Genetic Resources (Award HY202101)
    • Principle Award Recipient: QiliangLai
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: QiliangLai
  • Fujian Provincial Department of Science and Technology (Award 2020N0032)
    • Principle Award Recipient: QiufangZhang
© 2022 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005561
2022-11-02
2025-01-21
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/content/journal/ijsem/10.1099/ijsem.0.005561
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Portibacter marinus sp. nov., isolated from the sediment on the surface of plastics and proposal of a novel genus Neolewinella gen. nov. based on the genome-based phylogeny of the family Lewinellaceae
Int J Syst Evol Microbiol 72, 005561 (2022); https://doi.org/10.1099/ijsem.0.005561
/content/journal/ijsem/10.1099/ijsem.0.005561
/content/journal/ijsem/10.1099/ijsem.0.005561
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