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Volume 72, Issue 10

sp. nov., a pathogenic species of the genus isolated from soil in Puerto Rico Open Access

Abstract

Two spirochetes (designated strains LGVF01 and LGVF02) were isolated from soil samples in San Juan, Puerto Rico in HAN media after selection using a combination of ELISA, agar plating, and colony screening by Fluorescent Antibody Testing (FAT) and PCR for and . Isolates were helix-shaped, aerobic, fast-growing, and highly motile. Genome sequence analysis indicated that both strains should be classified as members of a novel species within the pathogenic (P1) clade of the genus . The average nucleotide identity between the two strains was 99.2 %, but below 93.2 % when compared to any previously described leptospiral species. Serotyping of strain LGVF02 indicates that it does not belong within any serogroup of suggesting it also represents a new serovar. Collectively, strains LGVF01 and LGVF02 represent a new species of pathogenic leptospires for which the name sp. nov. is proposed. The type strain is LGVF02 (=NVSL-LGVF02=KIT0302).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Leptospira , leptospirosis , pathogenic , soil and whole-genome sequencing
Funding
This study was supported by the:
  • Agricultural Research Service, USDA.
    • Principle Award Recipient: LuisG.V. Fernandes
  • Centers for Disease Control and Prevention (Award 6NU1ROT000006-01-04)
    • Principle Award Recipient: LuisG.V. Fernandes
  • FAPESP (Award 2017/06731-8 and 2019/20302-8)
    • Principle Award Recipient: LuisG.V. Fernandes
© Microbiology Society
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2022-10-19
2024-04-23
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Leptospira sanjuanensis sp. nov., a pathogenic species of the genus Leptospira isolated from soil in Puerto Rico
Int J Syst Evol Microbiol 72, 005560 (2022); https://doi.org/10.1099/ijsem.0.005560
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