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Abstract

Two spirochetes (designated strains LGVF01 and LGVF02) were isolated from soil samples in San Juan, Puerto Rico in HAN media after selection using a combination of ELISA, agar plating, and colony screening by Fluorescent Antibody Testing (FAT) and PCR for and . Isolates were helix-shaped, aerobic, fast-growing, and highly motile. Genome sequence analysis indicated that both strains should be classified as members of a novel species within the pathogenic (P1) clade of the genus . The average nucleotide identity between the two strains was 99.2 %, but below 93.2 % when compared to any previously described leptospiral species. Serotyping of strain LGVF02 indicates that it does not belong within any serogroup of suggesting it also represents a new serovar. Collectively, strains LGVF01 and LGVF02 represent a new species of pathogenic leptospires for which the name sp. nov. is proposed. The type strain is LGVF02 (=NVSL-LGVF02=KIT0302).

Funding
This study was supported by the:
  • Agricultural Research Service, USDA.
    • Principle Award Recipient: LuisG.V. Fernandes
  • Centers for Disease Control and Prevention (Award 6NU1ROT000006-01-04)
    • Principle Award Recipient: LuisG.V. Fernandes
  • FAPESP (Award 2017/06731-8 and 2019/20302-8)
    • Principle Award Recipient: LuisG.V. Fernandes
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2022-10-19
2024-12-07
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