1887

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Volume 72, Issue 9

sp. nov., a novel actinobacterium isolated from lake sediment No Access

Abstract

A novel actinobacterium, designated strain H8750, was isolated from sediment sampled at Lugu Lake, southwest PR China and its polyphasic taxonomy was studied. Strain H8750 produced well-developed substrate mycelium, and formed club-shaped and hooked structures borne on the tip of the aerial mycelia. It contained -diaminopimelic, glucose, ribose and madurose in whole-cell hydrolysates. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). The phospholipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, unidentified phospholipids and unidentified aminophospholipids. The major fatty acid (>10 %) were 9 C, iso-C and C. The DNA G+C content was 69.7 mol% based on the whole genome sequence. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences indicated that strain H8750 was closely related to JCM 10982 (98.0 %), JCM 10983 (97.9 %) and DSM 45867 (97.8 %) and formed a monophyletic clade within the genus in the phylogenetic trees. The average nucleotide identity and digital DNA–DNA hybridization values between strain H8750 and its closely related species were 79.8~87.2 % and 25.9~28.0 %, respectively, which showed that it belonged to a distinct species. Furthermore, the morphological and phenotypic characteristics allowed the isolate to be differentiated from its closely related species. Therefore, it is concluded that strain H8750 can be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H8750 (=JCM 34849=CICC 25116). Moreover, based on the gene prediction results, strain H8750 may have the genetic potential to synthesize many new secondary metabolites, which further increases its bioactive value.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Acrocarpospora catenulata sp. nov. and polyphasic taxonomy
Funding
This study was supported by the:
  • start-up fund of introducing talent of Chengdu University (Award No. 20181915049)
    • Principle Award Recipient: ChaolanLiu
  • Special Program for Basic Research of the Ministry of Science and 568 Technology, China (Award No. 2017FY100301)
    • Principle Award Recipient: YiwenChu
  • Sichuan Science and Technology Program (Award No. 2020YJ0276)
    • Principle Award Recipient: ChaolanLiu
© 2022 The Authors
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2022-09-21
2024-04-19
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Acrocarpospora catenulata sp. nov., a novel actinobacterium isolated from lake sediment
Int J Syst Evol Microbiol 72, 005526 (2022); https://doi.org/10.1099/ijsem.0.005526
/content/journal/ijsem/10.1099/ijsem.0.005526
/content/journal/ijsem/10.1099/ijsem.0.005526
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