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Abstract

-like isolates, strains 27fs60, 30fs61 and 30cs62, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy. Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32–37 °C (with an optimum at 32 °C) on buffered charcoal–yeast extract agar with -cysteine, glycine–vancomycin–polymyxin B–cycloheximide agar and Wadowsky–Yee medium agar. The strains showed positive reactions for oxidase, hippurate and gelatinase and a weakly positive reaction for catalase. Based on the EUCAST cut-off, strain 30cs62 was resistant to ciprofloxacin (5 mg l). The and gene sequences of the three strains showed close matches to those of ATCC 49507 with similarity values of 98.2 and 94.5 %, respectively. Whole genome sequencing of the three strains was performed, resulting in G+C contents of 39.0, 39.1 and 39.0 mol%, respectively. The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % . NCTC 12376, 91.45 % . ATCC 49507 and 91.45 % . ATCC 49507, respectively. The digital DNA–DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic species ( ATCC 49507, ≤40.10 % DNA–DNA relatedness). The concatenated phylogenetic tree based on 16S , and genes, shows a close relationship with ATCC 49507. The results obtained confirm the status of an independent species. The name proposed for this species is sp. nov. with 30cs62 (=ATCC TSD-262=DSM 112526) as the type strain.

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2022-09-29
2024-05-27
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