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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 72, Issue 9

sp. nov., isolated from a sheep with respiratory disease Free

Abstract

A novel Gram-stain-negative, aerobic, coccus-shaped bacteria, designated ZY201115, was isolated from the nasal cavity of a sheep with respiratory disease in Yunnan Province, south-west China, and its taxonomic affiliation was studied by applying a polyphasic approach. The strain grew at 18–41 °C (optimum, 37 °C), at pH 6.0–9.0 (optimum, pH 8.0) and in 0.5–3.0% (w/v) NaCl (optimum, 1.0 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain is affiliated to the genus with highest similarity to ATCC 10900 (96.6 %). Phylogenomic analysis based on 811 single-copy genes also indicated that the strain represents a novel species in the genus and formed a deep and separated clade with NCTC 10293. The highest genomic orthologous average nucleotide identity and digital DNA–DNA hybridization values between the strain and the type strains in the genus were 73.7% ( NCTC 10293) and 25.3% ( CCUG 350), respectively. The G+C content of the complete genome sequence was 42.1 mol%. The predominant fatty acids (>5 %) were C ω9, C ω8, C3OH and summed feature 3 (C ω7 and/or C ω6). The major polar lipids were phosphatidylglycerol, cardiolipin, monolysocardiolipin, phosphatidylethanolamine and hemibismonoacylglycerophosphate. The major respiratory quinone was CoQ-8. On the basis of the results of phylogenetic, phenotypic and chemotaxonomic characterizations, strain ZY201115 clearly represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ZY201115 (=CCTCC AB 2021473=CCUG 75922).

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Keyword(s): Moraxella nasovis , respiratory disease , sheep , taxonomy and virulence factor
© 2022 The Authors
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2022-09-06
2025-12-12

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Moraxella nasovis sp. nov., isolated from a sheep with respiratory disease
Int J Syst Evol Microbiol 72, 005511 (2022); https://doi.org/10.1099/ijsem.0.005511
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