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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 8

sp. nov., a moderate halophile isolated from hypersaline sediments of the Qaidam Basin in Northwest China No Access

Abstract

A Gram-stain-positive, moderately halophilic, aerobic, endospore-forming, rod-shaped bacterium, designated strain DP4-553-S, was isolated from hypersaline sediment collected from the Dalangtan Playa in the Qaidam Basin, Northwest PR China. Growth occurred within 0–21.6% (w/v) NaCl (optimum 7.2%) at pH 5.5–9.0 (optimum pH 7.0) and at 4–45 °C (optimum 37 °C). Phylogeny based on 16S rRNA gene sequences indicated that strain DP4-553-S belonged to the genus , with high 16S rRNA gene sequence similarity to EN8d (99.5 %), JSM 102062 (99.4 %), SK-1 (99.3 %) and NHBX5 (98.3 %). The G+C content of the chromosomal DNA was 43.6 mol %. The average amino acid identity, average nucleotide identity and digital DNA–DNA hybridization values between strain DP4-553-S and the four close type strains were 71.2–93.3, 74.0–90.5 and 20.0–41.4 %, respectively. The whole genomic analysis showed that strain DP4-553-S constituted a different taxon separated from the recognized species. The major cellular fatty acids were anteiso-C, anteiso-C, iso-C, C and iso-C. The type strain contained cell-wall peptidoglycan based on diaminopimelic acid and possessed menaquinone-7 as the major respiratory isoprenoid quinone. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, phosphatidylcholine, aminophospholipid, aminolipid and seven unidentified phospholipids. The combined data from phenotypic and genotypic studies demonstrated that strain DP4-553-S represents a novel species of the genus , for which the name sp. nov. is proposed, the type strain is DP4-553-S (=MCCC 1K03838= KCTC 43250).

  • Received:
  • Accepted:
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Keyword(s): hypersaline sediment , Mars analog , moderate halophile , Qaidam Basin and Sediminibacillus
Funding
This study was supported by the:
  • Science and Technology Development Fund (Award 0005/2020/A1)
    • Principle Award Recipient: TingHuang
  • The Pre-research Project on Civil Aerospace Technologies of CNSA (Award 9007)
    • Principle Award Recipient: YiXu
  • Science and Technology Development Fund (Award 121/2017/A3)
    • Principle Award Recipient: LongXiao
  • National Natural Science Foundation of China (Award 41830214)
    • Principle Award Recipient: LongXiao
  • National Key Research and Development Program of China (Award 2018YFC0310705)
    • Principle Award Recipient: LinfengGong
  • The Pre-research Project on Civil Aerospace Technologies of CNSA (Award D020101)
    • Principle Award Recipient: LongXiao
© 2022 The Authors
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2022-08-24
2024-12-07
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Sediminibacillus dalangtanensis sp. nov., a moderate halophile isolated from hypersaline sediments of the Qaidam Basin in Northwest China
Int J Syst Evol Microbiol 71, 005501 (2021); https://doi.org/10.1099/ijsem.0.005501
/content/journal/ijsem/10.1099/ijsem.0.005501
/content/journal/ijsem/10.1099/ijsem.0.005501
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