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Abstract

A Gram-negative bacterial strain, G163CM, was isolated from the gut of the Asian emerald cockroach . The 16S rRNA gene sequence (1416 bp) of strain G163CM showed 99.22% similarity to CCM 8479 and CPO20170097. The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values of strain G163CM were 92.4, 48.8 and 95.7% to CCM 8479, and 93.3, 52.4 and 95.7% to CPO20170097. This strongly supports the designation of G163CM as representing a new species in the genus . Phylogenetic trees based on the alignment of 16S rRNA, multilocus sequence analysis of six single-copy genes (, , , , and ) and 107 core protein sequences consistently showed G163CM to be a member of the genus , closely related to CPO20170097. In contrast to CCM 8479 and CPO20170097, the genome of G163CM did not encode for proteins conferring resistance to antibiotics. However, all three genomes encoded a similar number of virulence factors and specialized metabolite biosynthetic proteins. The major fatty acids of strain G163CM were C (31.5 %), C ω7c (22.6 %), C cyclo (15.3 %) and C (6.5 %). Based on the polyphasic results, we conclude that strain G163CM represents a novel species of the genus and we propose the name sp. nov. with the type strain G163CM (=DSM 112648=CCM 9160).

Funding
This study was supported by the:
  • Hessisches Ministerium für Wissenschaft und Kunst
    • Principle Award Recipient: AndreasVilcinskas
  • Alexander von Humboldt-Stiftung
    • Principle Award Recipient: JuanGuzman
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/content/journal/ijsem/10.1099/ijsem.0.005497
2022-08-23
2024-05-20
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