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Volume 72, Issue 8

sp. nov., isolated from soil of Sichang Island, Thailand No Access

Abstract

During an investigation of rare actinobacteria, isolate SC076 was isolated from a soil sample collected from Sichang Island, Chonburi Province, Thailand. The strain showed the highest 16S rRNA gene similarity to DSM 43800 (98.6%) and DSM 44229 (98.6%). The zigzag morphology of the spore chain was observed on the aerial mycelia. Diaminopimelic acid was detected in the peptidoglycan. Whole-cell sugars contained rhamnose, ribose, mannose glucose and galactose. Polar lipids were phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, unidentified ninhydrin-positive glycolipid, unidentified glycolipid and four unidentified lipids. The menaquinones were MK-9(H), MK-9(H), MK-9(H) and MK-9(H). The predominant fatty acids were iso-C, iso-C and anteiso-C. The draft genome of SC076 was 8040245 bp with a G+C content of 72.5 mol%. The results of genomic analysis between strain SC076 and the related type strains showed that the digital DNA–DNA hybridization and average nucleotide identity values among the strains were 23.6-32.8% and 77.7–86.8 %, respectively, which are lower than the thresholds used to distinguish strains from others of the same species. Based on the taxonomic evidence, strain SC076 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is SC076 (=TBRC 14540=NBRC 115117).

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Keyword(s): antimicrobial activity , polyphasic taxonomy , rare actinobacteria , Saccharothrix and Sichang Island
Funding
This study was supported by the:
  • Faculty of Pharmaceutical Sciences, Chulalongkorn University
    • Principle Award Recipient: WongsakornPhongsopitanun
© 2022 The Authors
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2022-08-12
2024-04-25
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Saccharothrix obliqua sp. nov., isolated from soil of Sichang Island, Thailand
Int J Syst Evol Microbiol 72, 005479 (2022); https://doi.org/10.1099/ijsem.0.005479
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