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Volume 72, Issue 6

sp. nov., a novel actinobacterium isolated from sediment of karst cave Open Access

Abstract

A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11 was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China. The isolate grew optimally on R2A agar at 25 °C, pH 8.0 and with 0.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11 belonged to the genus and was most closely related to TGA (98.3 % sequence similarity), LC6 (97.7 %) and CCM1646 (97.1 %). Genome sequencing revealed a genome size of 4 073 119 bp and the genomic DNA G+C content was 66.16 mol%. Its DNA–DNA relatedness values with TGA, LC6 and CCM1646 were 23.0, 22.9 and 23.2 %, respectively. The main fatty acids were anteiso-C, anteiso-C and iso-C. The major respiratory quinone was MK-9(H). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, glycolipid, phosphatidylethanolamine, phosphatidylinositol and unidentified lipids. Thus, based on phylogenetic and phenotypic and chemotaxonomic data, strain PO-11 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain PO-11 (=CCTCC AB 2021070=LMG 32459).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinobacteria , Arthrobacter cavernae , karst cave , novel species , phylogenetic analysis and sediment
Funding
This study was supported by the:
  • Natural Science Foundation of Guizhou Province (Award Qiankehejichu [2019]1223)
    • Principle Award Recipient: ZhengFang
  • Mission on Nano Science and Technology (Award Grant No. [2019]5617, [2019]5655)
    • Principle Award Recipient: QingbeiWeng
  • Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province (Award (U1812401))
    • Principle Award Recipient: QingbeiWeng
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2024-04-23
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Arthrobacter cavernae sp. nov., a novel actinobacterium isolated from sediment of karst cave
Int J Syst Evol Microbiol 72, 005445 (2022); https://doi.org/10.1099/ijsem.0.005445
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