1887

Abstract

Two novel species, designated strains SYSU G04041 and SYSU G04536, were isolated from hot spring sediments collected in Yunnan, PR China. Phenotypic and chemotaxonomic analyses, and whole-genome sequencing were used to determine the taxonomic positions of the candidate strains. Phylogenetic analysis using 16S rRNA gene sequence indicated that strain SYSU G04041 showed the highest sequence similarity to A50-7-3 (97.5 %), and SYSU G04536 showed the highest sequence similarity to SGM-6 (98.2 %). The strains could be differentiated from other species of the genus by their distinct phenotypic and genotypic characteristics. Cells of strains SYSU G04041 and SYSU G04536 were aerobic, motile and Gram-stain-negative. Growth both occurred optimally at 45 °C and pH 7.0 for SYSU G04041 and SYSU G04536. In addition, the predominant respiratory quinone in both isolates was ubiquinone Q-8. The major fatty acids (>10 %) of strain SYSU G04041 were C, iso-C and iso-C, while the major fatty acids (>10 %) of strain SYSU G04536 were iso-C and iso-C. The main detected polar lipids in strains SYSU G04041 and SYSU G04536 included phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The G+C contents of the genomic DNA of strains SYSU G04041 and SYSU G04536 based on draft genomic sequences were 72.5 and 68.3 %, respectively. On the basis of phenotypic, genotypic and phylogenetic data, strains SYSU G04041 and SYSU G04536 represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed, with the type strains SYSU G04041 (=CGMCC 1.19366=KCTC 92228) and SYSU G04536 (=CGMCC 1.19367=KCTC 82839), respectively.

Funding
This study was supported by the:
  • Guangdong Basic and Applied Basic Research Foundation (Award 2019A1515110227)
    • Principle Award Recipient: LanLiu
  • Sun Yat-Sen University 'college students' innovation and entrepreneurship training program (Award 202210478)
    • Principle Award Recipient: Jun-YiDai
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2019FY100701)
    • Principle Award Recipient: Bao-ZhuFang
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: Jian-YuJiao
  • National Natural Science Foundation of China (Award 91951205)
    • Principle Award Recipient: Wen-JunLi
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2022-06-24
2024-05-29
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