1887

Abstract

Three yeast strains, DMKU-GTSP8-6, DMKU-GTSP8-14 and DMKU-JED8-73, were isolated from food waste in Thailand. Based on the phenotypic characteristics and sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, it was revealed that the three strains clustered with the / clade. These strains were distinguished from the closely related species CBS 750.85, CBS 192.55, CBS 780.96, CBS 8187 and CBS 517.90 by 11.4, 13.1, 11.9, 11.2 and 12.6 % sequence divergence in the D1/D2 domain and by 34.6, 34.5, 33.6, 33.2 and 34.9 % sequence divergence in the ITS region, respectively. The new species, which does not produce ascospores, is described as . The holotype of is TBRC 15056, and the isotypes are DMKU-GTSP8-14 and PYCC 9023.

Funding
This study was supported by the:
  • Graduate School Scholarship (Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University) (Award Graduate School Scholarship (Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University))
    • Principle Award Recipient: JemishaDudhat
  • Kasetsart University Research and Development Institute (Award FF(KU)18.64)
    • Principle Award Recipient: NantanaSrisuk
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2022-06-17
2024-10-08
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References

  1. Kurtzman CP, Fell J, Boekhout T. The Yeasts, a Taxonomic Study, 5th edn. Amsterdam: Elsevier; 2011
    [Google Scholar]
  2. Smith MT. Ribosomal gene phylogeny and species delimitation in Geotrichum and its teleomorphs. Stud Mycol 2004; 50:489–515
    [Google Scholar]
  3. Redhead SA, Malloch DW. The Endomycetaceae: new concepts, new taxa. Can J Bot 1977; 55:1701–1711 [View Article]
    [Google Scholar]
  4. de Hoog GS, Smith MT. Saprochaete Coker & Shanor ex DTS Wagner & Dawes (1970). In Fell J, Boekhout T. eds The Yeasts, a Taxonomic Study, 5th edn. Amsterdam: Elsevier; 2011 pp 1317–1327
    [Google Scholar]
  5. de Hoog GS, Smith MT. Magnusiomyces Zender (1977). In Kurtzman CP, Fell J, Boekhout T. eds The Yeasts, a Taxonomic Study, 5th edn. Amsterdam: Elsevier; 2011 pp 565–574
    [Google Scholar]
  6. de Hoog GS, Smith MT. Geotrichum Link: Fries (1832). In Kurtzman CP, Fell J, Boekhout T. eds The Yeasts, a Taxonomic Study, 5th edn. Amsterdam: Elsevier; 2011 pp 1279–1286
    [Google Scholar]
  7. Butler EE, Petersen LJ. Endomyces geotrichum a perfect state of Geotrichum candidum. Mycologia 1972; 64:365–374 [View Article] [PubMed]
    [Google Scholar]
  8. de Hoog GS GS, Smith MT, Guého E. Revision of the genus Geotrichum and its telemorphs. Centraalbureau voar Schimmelcultures 1986
    [Google Scholar]
  9. Shen X-X, Opulente DA, Kominek J, Zhou X, Steenwyk JL et al. Tempo and mode of genome evolution in the budding yeast subphylum. Cell 2018; 175:1533–1545 [View Article] [PubMed]
    [Google Scholar]
  10. Morel G. La levure Geotrichum candidum: taxonomie, biodiversité et génome Doctoral dissertation, Paris: 2012 p 11
    [Google Scholar]
  11. Desnos-Ollivier M, Blanc C, Garcia-Hermoso D, Hoinard D, Alanio A et al. Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases. J Clin Microbiol 2014; 52:2196–2198 [View Article] [PubMed]
    [Google Scholar]
  12. Bader O, Weig M, Taverne-Ghadwal L, Lugert R, Gross U et al. Improved clinical laboratory identification of human pathogenic yeasts by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Microbiol Infect 2011; 17:1359–1365 [View Article] [PubMed]
    [Google Scholar]
  13. Giacchino M, Chiapello N, Bezzio S, Fagioli F, Saracco P et al. Aspergillus galactomannan enzyme-linked immunosorbent assay cross-reactivity caused by invasive Geotrichum capitatum . J Clin Microbiol 2006; 44:3432–3434 [View Article] [PubMed]
    [Google Scholar]
  14. Lohmann C, Sabou M, Moussaoui W, Prévost G, Delarbre J-M et al. Comparison between the Biflex III-Biotyper and the Axima-SARAMIS systems for yeast identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2013; 51:1231–1236 [View Article] [PubMed]
    [Google Scholar]
  15. Kaplan E, Al-Hatmi AMS, Ilkit M, Gerrits van den Ende AHG, Hagen F et al. Molecular diagnostics of arthroconidial yeasts, frequent pulmonary opportunists. J Clin Microbiol 2018; 56:e01427-17 [View Article] [PubMed]
    [Google Scholar]
  16. García-Ruiz JC, López-Soria L, Olazábal I, Amutio E, Arrieta-Aguirre I et al. Invasive infections caused by Saprochaete capitata in patients with haematological malignancies: report of five cases and review of the antifungal therapy. Rev Iberoam Micol 2013; 30:248–255 [View Article] [PubMed]
    [Google Scholar]
  17. Girmenia C, Pagano L, Martino B, D’Antonio D, Fanci R et al. Invasive infections caused by Trichosporon species and Geotrichum capitatum in patients with hematological malignancies: a retrospective multicenter study from Italy and review of the literature. J Clin Microbiol 2005; 43:1818–1828 [View Article] [PubMed]
    [Google Scholar]
  18. Sakpuntoon V, Angchuan J, Boontham W, Khunnamwong P, Boonmak C et al. Grease waste as a reservoir of lipase-producing yeast and description of Limtongella siamensis gen. nov., sp. nov. Microorganisms 2019; 8:E27 [View Article] [PubMed]
    [Google Scholar]
  19. Limtong S, Yongmanitchai W, Tun MM, Kawasaki H, Seki T. Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand. Int J Syst Evol Microbiol 2007; 57:419–422 [View Article] [PubMed]
    [Google Scholar]
  20. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article] [PubMed]
    [Google Scholar]
  21. Kurtzman CP. Phylogenetic circumscription of Saccharomyces, Kluyveromyces and other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora . FEMS Yeast Res 2003; 4:233–245 [View Article] [PubMed]
    [Google Scholar]
  22. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999; 45:95–98
    [Google Scholar]
  23. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article] [PubMed]
    [Google Scholar]
  24. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article] [PubMed]
    [Google Scholar]
  25. Kurtzman CP, Fell J, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell J, Boekhout T. eds The Yeasts, a Taxonomic Study, 5th edn. Amsterdam: Elsevier; 2011 pp 87–110
    [Google Scholar]
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