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Volume 72, Issue 5

sp. nov., a hydrocarbon-utilizing bacterium isolated from the Mariana Trench No Access

Abstract

Two strains, TMPB967 and TTPB476, were isolated from two different locations in the Mariana Trench. Cells of strains TMPB967 and TTPB476 were Gram-negative, curved rod-shaped (0.35–0.6 µm×2–4 µm) with flagella. Both strains were catalase- and oxidase-positive. Strains TMPB967 and TTPB476 could grow at 4–37 °C (optimum, 37 °C), at pH 6–9 (optimum, pH 6–7) and in the presence of 0–8 % (w/v) NaCl (optimum, 5 %). Both strains could grow with tetradecane or hexadecane as the sole carbon source. The predominant isoprenoid quinone was ubiquinone 9. The major cellular fatty acids of strains TMPB967 and TTPB476 were C 9, C and summed feature 3 (C 7 or 6). The polar lipid profile included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown aminolipid. The DNA G+C contents of strains TMPB967 and TTPB476 were 53.1 and 53.0 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the most closely related validly published species were IMCC1826 (97.1 % similarity) and MIL-1 (95.9 % similarity). Digital DNA–DNA hybridization results of strain TMPB967 with TTPB476, IMCC1826 and MIL-1 were 99.9, 20.9 and 20.2 %, respectively. Average nucleotide identity results of strain TMPB967 with TTPB476, IMCC1826 and MIL-1 were 100, 75.8 and 72.0 %, respectively. On the basis of the phenotypic, chemotaxonomic and molecular features, strains TMPB967 and TTPB476 belong to a novel species within the genus , for which the name sp. nov. is proposed. The type strain is TMPB967 (=KCTC 82621=MCCC 1K05476).

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Keyword(s): alkB , Mariana Trench , petroleum hydrocarbons and Thalassolituus alkanivorans sp. nov.
Funding
This study was supported by the:
  • Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province (Award 19DZ2282100)
    • Principle Award Recipient: Zhe-XueQuan
  • National Natural Science Foundation of China (Award 31870109, 42176101)
    • Principle Award Recipient: Zhe-XueQuan
  • National Key R&D Program of China (Award 2018YFC0310600)
    • Principle Award Recipient: Zhe-XueQuan
© 2022 The Authors
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2022-05-31
2024-04-20
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Thalassolituus alkanivorans sp. nov., a hydrocarbon-utilizing bacterium isolated from the Mariana Trench
Int J Syst Evol Microbiol 72, 005404 (2022); https://doi.org/10.1099/ijsem.0.005404
/content/journal/ijsem/10.1099/ijsem.0.005404
/content/journal/ijsem/10.1099/ijsem.0.005404
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