1887

Abstract

An anaerobic bacterial strain, designated as NSJ-90, was isolated from the faeces of a healthy adult in China. Cells of strain NSJ-90 were Gram-stain-negative, non-motile, non-spore-forming and rod-shaped. Based on 16S rRNA gene sequence analysis, strain NSJ-90 belonged to the genus and was phylogenetically closely related to YIT 12056 (16S rRNA gene identity was 97.04 %). The DNA G+C content of strain NSJ-90 was 44.85 mol% (calculated from the genome). The average nucleotide identity between strain NSJ-90 and YIT 12056 was 87.60 %. The major cellular fatty acids (>10 %) of strain NSJ-90 were iso-C, anteiso-C and iso-C 3-OH. Menaquinone-10 was detected as the respiratory quinone. The major products of glucose fermentation were acetic, propionic and isovaleric acids. Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, we propose that strain NSJ-90 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NSJ-90 (=CGMCC 1.17886=KCTC 25305).

Funding
This study was supported by the:
  • Ministry of Science and Technology of the People's Republic of China (Award Grant No. 2019YFA0905601)
    • Principle Award Recipient: ApplicableNot
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.005397
2022-05-30
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/72/5/ijsem005397.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.005397&mimeType=html&fmt=ahah

References

  1. Shah HN, Collins MD. Genus Bacteroides. A chemotaxonomical perspective. J Appl Bacteriol 1983; 55:403–416 [View Article]
    [Google Scholar]
  2. Shah HN, Collins MD. Fatty acid and isoprenoid quinone composition in the classification of Bacteroides melaninogenicus and related taxa. J Appl Bacteriol 1980; 48:75–87 [View Article] [PubMed]
    [Google Scholar]
  3. Mahowald MA, Rey FE, Seedorf H, Turnbaugh PJ, Fulton RS et al. Characterizing a model human gut microbiota composed of members of its two dominant bacterial phyla. Proc Natl Acad Sci U S A 2009; 106:5859–5864 [View Article] [PubMed]
    [Google Scholar]
  4. Nishiyama T, Ueki A, Kaku N, Watanabe K, Ueki K. Bacteroides graminisolvens sp. nov., a xylanolytic anaerobe isolated from a methanogenic reactor treating cattle waste. Int J Syst Evol Microbiol 2009; 59:1901–1907 [View Article] [PubMed]
    [Google Scholar]
  5. Ueki A, Abe K, Ohtaki Y, Kaku N, Watanabe K et al. Bacteroides paurosaccharolyticus sp. nov., isolated from a methanogenic reactor treating waste from cattle farms. Int J Syst Evol Microbiol 2011; 61:448–453 [View Article] [PubMed]
    [Google Scholar]
  6. Wexler AG, Goodman AL. An insider’s perspective: Bacteroides as a window into the microbiome. Nat Microbiol 2017; 2:17026 [View Article] [PubMed]
    [Google Scholar]
  7. Sun F, Zhang Q, Zhao J, Zhang H, Zhai Q et al. A potential species of next-generation probiotics? The dark and light sides of Bacteroides fragilis in health. Food Res Int 2019; 126:108590 [View Article] [PubMed]
    [Google Scholar]
  8. Tan H, Zhao J, Zhang H, Zhai Q, Chen W. Novel strains of Bacteroides fragilis and Bacteroides ovatus alleviate the LPS-induced inflammation in mice. Appl Microbiol Biotechnol 2019; 103:2353–2365 [View Article] [PubMed]
    [Google Scholar]
  9. Wang C, Zhao J, Zhang H, Lee Y-K, Zhai Q et al. Roles of intestinal bacteroides in human health and diseases. Crit Rev Food Sci Nutr 2021; 61:3518–3536 [View Article] [PubMed]
    [Google Scholar]
  10. Yu SY, Kim J-S, Oh BS, Park S-H, Kang SW et al. Bacteroides faecalis sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 2019; 69:3824–3829 [View Article] [PubMed]
    [Google Scholar]
  11. Sorokin DY. Is there a limit for high-pH life?. Int J Syst Evol Microbiol 2005; 55:1405–1406 [View Article] [PubMed]
    [Google Scholar]
  12. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article] [PubMed]
    [Google Scholar]
  13. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
    [Google Scholar]
  14. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article] [PubMed]
    [Google Scholar]
  15. Wu GD, Chen J, Hoffmann C, Bittinger K, Chen Y-Y et al. Linking long-term dietary patterns with gut microbial enterotypes. Science 2011; 334:105–108 [View Article] [PubMed]
    [Google Scholar]
  16. Zuo G. CVTree: A parallel alignment-free phylogeny and taxonomy tool based on composition vectors of genomes. Genomics Proteomics Bioinformatics 2021; 19:662–667 [View Article]
    [Google Scholar]
  17. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article]
    [Google Scholar]
  18. Athalye M, Noble WC, Minnikin DE. Analysis of cellular fatty acids by gas chromatography as a tool in the identification of medically important coryneform bacteria. J Appl Bacteriol 1985; 58:507–512 [View Article]
    [Google Scholar]
  19. Minnikin DE, Abdolrahimzadeh H. Effect of pH on the proportions of polar lipids, in chemostat cultures of Bacillus subtilis. J Bacteriol 1974; 120:999–1003 [View Article]
    [Google Scholar]
  20. Miller LT. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 1982; 16:584–586 [View Article]
    [Google Scholar]
  21. Watanabe Y, Nagai F, Morotomi M, Sakon H, Tanaka R. Bacteroides clarus sp. nov., Bacteroides fluxus sp. nov. and Bacteroides oleiciplenus sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 2010; 60:1864–1869 [View Article] [PubMed]
    [Google Scholar]
  22. Mayberry WR, Lambe DW, Ferguson KP. Identification of bacteroides species by cellular fatty acid profiles. Int J Syst Bacteriol 1982; 32:21–27 [View Article]
    [Google Scholar]
  23. Collins MD, Green PN. Isolation and characterization of a novel coenzyme Q from some methane-oxidizing bacteria. Biochem Biophys Res Commun 1985; 133:1125–1131 [View Article] [PubMed]
    [Google Scholar]
  24. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article] [PubMed]
    [Google Scholar]
  25. Shin Y, Park S-J, Paek J, Kim J-S, Rhee M-S et al. Bacteroides koreensis sp. nov. and Bacteroides kribbi sp. nov., two new members of the genus Bacteroides. Int J Syst Evol Microbiol 2017; 67:4352–4357 [View Article] [PubMed]
    [Google Scholar]
  26. Sakamoto M, Ohkuma M. Bacteroides reticulotermitis sp. nov., isolated from the gut of a subterranean termite (Reticulitermes speratus). Int J Syst Evol Microbiol 2013; 63:691–695 [View Article] [PubMed]
    [Google Scholar]
  27. Bakir MA, Kitahara M, Sakamoto M, Matsumoto M, Benno Y. Bacteroides intestinalis sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 2006; 56:151–154 [View Article] [PubMed]
    [Google Scholar]
  28. Lan PTN, Sakamoto M, Sakata S, Benno Y. Bacteroides barnesiae sp. nov., Bacteroides salanitronis sp. nov. and Bacteroides gallinarum sp. nov., isolated from chicken caecum. Int J Syst Evol Microbiol 2006; 56:2853–2859 [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.005397
Loading
/content/journal/ijsem/10.1099/ijsem.0.005397
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error