A Gram-stain-negative, aerobic, non-motile, rod-shaped or occasionally filamentous-shaped bacterial strain, designated 5GH13-10T, was isolated from greenhouse soil sampled in Yeoju-si, Republic of Korea. Colonies were milky-coloured, round and convex, and catalase- and oxidase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 5GH13-10T was related to the genus Arachidicoccus and had highest 16S rRNA gene sequence identity with Arachidicoccus rhizosphaerae Vu-144T (98.4 %). The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c). The predominant quinone was menaquinone MK-7, and the polar lipids were composed of phosphatidylethanolamine, one unidentified aminolipid, three unidentified aminophospholipids, one unidentified phospholipid and five unidentified lipids. The genomic DNA G+C content of strain 5GH13-10T was 43.8 mol%. The average nucleotide identity values between strain 5GH13-10T and the closely related species Arachidicoccus ginsenosidivorans Gsoil 809T, Arachidicoccus rhizosphaerae Vu-144T and Archidicoccus soli KIS59-12T were 74.86, 74.74 and 69.52 %, and the digital DNA-DNA hybridization values were 20.0, 19.8 and 18.6 %, respectively. Combined phenotypic, phylogenetic and genomic data demonstrated that strain 5GH13-10T is representative of a novel species of the genus Arachidicoccus, for which we propose the name Arachidicoccus terrestris sp. nov. (type strain 5GH13-10T=KACC 18014T=NBRC 113162T).
MadhaiyanM, PoonguzhaliS, SenthilkumarM, PragatheswariD, LeeJ-S et al.Arachidicoccus rhizosphaerae gen. nov., sp. nov., a plant-growth-promoting bacterium in the family Chitinophagaceae isolated from rhizosphere soil. Int J Syst Evol Microbiol2015; 65:578–586 [View Article]
KämpferP, LoddersN, FalsenE. Hydrotalea flava gen. nov., sp. nov., a new member of the phylum Bacteroidetes and allocation of the genera Chitinophaga, Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas and Hydrotalea to the family Chitinophagaceae fam. nov. Int J Syst Evol Microbiol2011; 61:518–523 [View Article]
SiddiqiMZ, ShafiSM, ImWT. Complete genome sequencing of Arachidicoccus ginsenosidimutans sp. nov., and its application for production of minor ginsenosides by finding a novel ginsenoside-transforming β-glucosidase. RSC Adv2017; 7:46745–46759 [View Article]
LaneDJ. 16S/23S rRNA sequencing. In StackebrandtE, GoodfellowM. eds Nucleic Acid Techniques in Bacterial Systematics New York, FL: John Wiley and Sons; 1991 pp 115–175
YoonS-H, HaS-M, KwonS, LimJ, KimY et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol2017; 67:1613–1617 [View Article] [PubMed]
TamuraK. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+C-content biases. Mol Biol Evol1992; 9:678–687 [View Article] [PubMed]
AuchAF, von JanM, KlenkH-P, GökerM. Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci2010; 2:117–134 [View Article] [PubMed]
ChunJ, OrenA, VentosaA, ChristensenH, ArahalDR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol2018; 68:461–466 [View Article] [PubMed]
OverbeekR, OlsonR, PuschGD, OlsenGJ, DavisJJ et al. The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST). Nucleic Acids Res2014; 42:D206–14 [View Article] [PubMed]
KatohA, UenoharaK, AkitaM, HashimotoT. Early steps in the biosynthesis of NAD in Arabidopsis start with aspartate and occur in the plastid. Plant Physiol2006; 141:851–857 [View Article] [PubMed]
ZhuF, PeñaM, BennettGN. Metabolic engineering of Escherichia coli for quinolinic acid production by assembling L-aspartate oxidase and quinolinate synthase as an enzyme complex. Metab Eng2021; 67:164–172 [View Article] [PubMed]
SmibertRM, KriegNR. Phenotypic characterization. In GerhardtP, MurrayR, WW, KriegN. eds Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp 607–654
SasserM. Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE, USA: Microbial ID Inc; 1990
MinnikinDE, O’DonnellAG, GoodfellowM, AldersonG, AthalyeM et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods1984; 2:233–241 [View Article]