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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 5

sp. nov., sp. nov., sp. nov. and sp. nov., four extremely halophilic archaea isolated from salt mine, saline soil and salt lake No Access

Abstract

Four halophilic archaeal strains, YPL8, SLN56, LT61 and KZCA68, were isolated from a salt mine, saline soil and a salt lake located in different regions of China. Sequence similarities of 16S rRNA and genes among strains YPL8, SLN56, LT61 and the current members of were 94.1–98.2 % and 89.3–95.1 %, respectively, while these values among strain KZCA68 and the current members of were 97.2–97.4 % and 91.7–91.9 %, respectively. The average nucleotide identity, DNA–DNA hybridization and average amino acid identity values among these four strains and their closely related species were all lower than the threshold values for species boundary. All four strains were unable to hydrolyse casein, gelatin, or Tween 80. Strain YPL8 contained phosphatidic acid (PA), phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1), disulfated mannosyl glucosyl diether (S-DGD) and sulfated mannosyl glucosyl diether-phosphatidic acid (S-DGD-PA). Strain SLN56 contained PA, PG, phosphatidylglycerol sulphate (PGS), PGP-Me, S-DGD-1, S-DGD and S-DGD-PA. Strain LT61 contained PA, PG, PGS, PGP-Me, S-DGD-1 and S-DGD. The phospholipids of strain KZCA68 were PA, PG and PGP-Me. These results showed that strains YPL8 (=CGMCC 1.13883=JCM 31181), SLN56 (=CGMCC 1.14945=JCM 30832) and LT61 (=CGMCC 1.14942=JCM 30668) represent novel species of the genus , for which the names, sp. nov., sp. nov. and sp. nov. are proposed. Strain KZCA68 (=CGMCC 1.17211=JCM 34158) represents a novel species of , for which the name sp. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): halophilic archaea , Haloterrigena , Natrinema , novel species , saline soil , salt lake and salt mine
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32070003)
    • Principle Award Recipient: Heng-LinCui
© 2022 The Authors
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2022-05-26
2024-04-18
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Natrinema halophilum sp. nov., Natrinema salinisoli sp. nov., Natrinema amylolyticum sp. nov. and Haloterrigena alkaliphila sp. nov., four extremely halophilic archaea isolated from salt mine, saline soil and salt lake
Int J Syst Evol Microbiol 72, 005385 (2022); https://doi.org/10.1099/ijsem.0.005385
/content/journal/ijsem/10.1099/ijsem.0.005385
/content/journal/ijsem/10.1099/ijsem.0.005385
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