Mycobacterium senriense sp. nov., a slowly growing, non-scotochromogenic species, isolated from sputum of an elderly man

Yuko Abe; Kiyoharu Fukushima; Yuki Matsumoto; Takayuki Niitsu; Hiroshi Nabeshima; Yasuharu Nagahama; Eri Akiba; Kako Haduki; Haruko Saito; Tadayoshi Nitta; Akira Kawano; Michio Tanaka; Takanori Matsuki; Daisuke Motooka; Kazuyuki Tsujino; Keisuke Miki; Shota Nakamura; Tetsuya Iida; Hiroshi Kida

doi:10.1099/ijsem.0.005378

Volume 72, Issue 5

Research Article

Mycobacterium senriense sp. nov., a slowly growing, non-scotochromogenic species, isolated from sputum of an elderly man

Yuko Abe¹, Kiyoharu Fukushima^1,2,3, Yuki Matsumoto⁴, Takayuki Niitsu¹, Hiroshi Nabeshima^3,5, Yasuharu Nagahama^3,5, Eri Akiba², Kako Haduki², Haruko Saito², Tadayoshi Nitta², Akira Kawano², Michio Tanaka⁶, Takanori Matsuki², Daisuke Motooka⁴, Kazuyuki Tsujino², Keisuke Miki², Shota Nakamura⁴, Tetsuya Iida⁶ and Hiroshi Kida²
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Affiliations: ¹ Department of Respiratory Medicine and Clinical Immunology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, Japan ² Department of Respiratory Medicine, National Hospital Organization Osaka Toneyama Medical Center, 5-1-1 Toneyama Toyonaka, Osaka, Japan ³ Laboratory of Host Defense, World Premier Institute Immunology Frontier Research Center (WPI-IFReC), Osaka University, 3-1 Yamadaoka, Suita, Osaka, Japan ⁴ Department of Infection Metagenomics, Genome Information Research Center, Research Institute for Microbial Diseases (RIMD), Osaka University, 3-1 Yamadaoka, Suita, Osaka, Japan ⁵ Fujii Memorial Research Institute, Otsuka Pharmaceutical Company, Ltd., 520-0106 Shiga, Japan ⁶ Pathogenic Microbes Repository Unit, Research Institute for Microbial Diseases (RIMD), Osaka University, 3-1 Yamadaoka, Suita, Osaka, Japan
*Correspondence: Kiyoharu Fukushima, [email protected]
Published: 23 May 2022 https://doi.org/10.1099/ijsem.0.005378

Abstract

A slowly growing mycobacteria, identified as strain TY59T, was isolated from sputum of an elderly man with pneumonia. Sequencing of the 16S rRNA gene indicated that this strain was similar to members of the Mycobacterium avium complex and closely related species. Strain TY59T has highest 16S rRNA gene sequence similarities to the type strains of Mycobacterium colombiense (99.80 % sequence similarity), Mycobacterium vulneris (99.74 %), Mycobacterium timonense (99.54 %), Mycobacterium avium subsp. avium (99.54 %) and Mycobacterium avium subsp. silvaticum (99.54 %). Analysis of the internal transcribed spacer (ITS) and DNA-directed RNA polymerase subunit beta (rpoB) sequences gave similar results to the 16S rRNA gene analysis. The closest species to strain TY59T were M. colombiense and M. vulneris with 97.90–98.25 % identity in ITS and 96.4–96.6 % in rpoB. The strain's 65 kDa heat shock protein (hsp65) gene was different from those of M. vulneris , M. colombiense and M. avium subsp. silvaticum with 72.4–74.2 % identity. Average nucleotide identity results showed a 93.4 % match to M. vulneris as the maximum value. Phenotypically, the non-chromogenicity, rough colonies, growth at 42 °C, negative results for nitrate reduction, β-glucosidase and Tween 80 hydrolysis, and positive results for catalase activity set this strain apart from closely related species. We propose that Mycobacterium senriense sp. nov. is a novel species of slowly growing mycobacteria. The type strain is TY59T (RIMD 1371001T=CIP 111917T).

Received: 17/08/2021
Accepted: 28/03/2022
Published Online: 23/05/2022

Keyword(s): mycobacteria , Mycobacterium avium complex and novel

Funding

This study was supported by the:

The Japan Intractable Diseases (Nanbyo) Research Foundation (Award 2020B02)
- Principle Award Recipient: KiyoharuFukushima
Japan Society for the Promotion of Science (Award JP21K08196)
- Principle Award Recipient: HiroshiKida
Japan Society for the Promotion of Science (Award JP21K16118)
- Principle Award Recipient: KiyoharuFukushima
GlaxoSmithKline foundation (Award A-32)
- Principle Award Recipient: KiyoharuFukushima
Japan Agency for Medical Research and Development (Award JP21lm0203007)
- Principle Award Recipient: KiyoharuFukushima
Japan Agency for Medical Research and Development (Award JP21fk0108129h0702)
- Principle Award Recipient: HiroshiKida

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Mycobacterium senriense sp. nov., a slowly growing, non-scotochromogenic species, isolated from sputum of an elderly man

Int J Syst Evol Microbiol 72, 005378 (2022); https://doi.org/10.1099/ijsem.0.005378

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Volume 72, Issue 5

Research Article

Mycobacterium senriense sp. nov., a slowly growing, non-scotochromogenic species, isolated from sputum of an elderly man

Abstract

Funding

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