1887

Abstract

Cells of members of the family , phylum , are generally obligate anaerobic rods. Strains D2Q-14 and D2Q-11 were isolated from sediment of the saline lake Manisi in the Xinjiang Uygur Autonomous Region, PR China. In this study, we employed a polyphasic approach and whole genome analysis of the two isolates. Cells of both isolates were Gram-stain-positive rods that were motile by means of flagella and could utilize sulphate, thiosulphate, elemental sulphur and nitrate as electron acceptors. Phylogenetic analyses based on 16S rRNA gene and whole genome sequences indicated that strains D2Q-14 and D2Q-11 constituted a coherent cluster affiliated to the family . In addition, genome analysis revealed that strain D2Q-14harboured one nonribosomal peptide synthetase gene cluster, making up 1.4 % of the entire genome. The genome-based analysis, including average nucleotide identity, average amino acid identity and DNA–DNA hybridization, biochemical, phenotypic and chemotaxonomic characterization, indicated that strains D2Q-14 and D2Q-11 represented two novel species of a novel genus in the family , for which we propose the names gen. nov., sp. nov. and sp. nov., with the type strains D2Q-14 (=KCTC 15986=MCCC 1K04634) and D2Q-11 (=KCTC 15985=MCCC 1K04391), respectively.

Funding
This study was supported by the:
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: RanZhang
  • National Natural Science Foundation of China (Award 320000001)
    • Principle Award Recipient: NotApplicable
  • Natural Science Foundation of Zhejiang Province (Award LQ19C010006)
    • Principle Award Recipient: RanZhang
  • Science and Technology Basic Resources Investigation Program of China (Award 2017FY100300)
    • Principle Award Recipient: RanZhang
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2022-05-13
2022-05-27
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