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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 5

Polar lipid characterization and description of sp. nov., isolated from high CO-containing atmosphere and emended descriptions of the genus , and the species , , , , , , and No Access

Abstract

Modified atmosphere (MA) packaging plays an important role in improving food quality and safety. By using different gas mixtures and packaging materials the shelf life of fresh produce can significantly be increased. A Gram-negative-staining, rod-shaped, orange-pigmented strain DH-B6, has been isolated from MA packed raw pork sausage (20% CO, 80% O). The strain produced biofilms and showed growth at high CO levels of up to 40%. Complete 16S rRNA gene and whole-genome sequences revealed that strain DH-B6 belongs to the genus , being closely related to strain DSM 16777 (98.4%), followed by NCTC11432 (98.3%) and KC1864 (98.2%). Average nucleotide identity value between DH-B6 and DSM 16777 was 81.1% and digital DNA–DNA hybridisation was 24.9%, respectively. The DNA G+C content was 35.51 mol%. Chemotaxonomical analysis revealed the presence of the rare glycine lipid cytolipin, the serine-glycine lipid flavolipin and the sulfonolipid sulfobacin A, as well as phosphatidylethanolamine, monohexosyldiacylglycerol and ornithine lipid, including the hydroxylated forms. Major fatty acids were iC (50.7%) and iC cis 9 (28.7%), followed by iC 2-OH (7.0%) and iC 3-OH (6.2%). The isolated strain contained MK-6 as the only respiratory quinone and flexirubin-like pigments were detected as the major pigments. Based on the phenotypic, chemotaxonomic and phylogenetic characteristics, the strain DH-B6 (=DSM 110542=LMG 31915) represents a novel species of the genus , for which the name sp. nov. is proposed. Emended descriptions of the genus and eight species of this genus based on polar lipid characterisation are also proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): carbon dioxide , Chryseobacterium , food microbiology , modified atmosphere packaging , novel species and polar lipids
© 2022 The Authors
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2022-05-05
2024-04-16
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http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.005372
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Polar lipid characterization and description of Chryseobacterium capnotolerans sp. nov., isolated from high CO2-containing atmosphere and emended descriptions of the genus Chryseobacterium, and the species C. balustinum, C. daecheongense, C. formosense, C. gleum, C. indologenes, C. joostei, C. scophthalmum and C. ureilyticum
Int J Syst Evol Microbiol 72, 005372 (2022); https://doi.org/10.1099/ijsem.0.005372
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