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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 5

sp. nov., sp. nov. and sp. nov., isolated from freshwater, rusty iron and forsythia flower No Access

Abstract

Three novel strains, designated as HMF5036, HMF5335 and HMF5405, were isolated from freshwater, rusty iron and forsythia flower, in Yong-in, Republic of Korea, respectively. They were Gram-stain-negative, facultatively anaerobic, non-motile, reddish-pigmented and rod-shaped bacteria. The predominant fatty acids of three strains were C 5 and summed feature 3 (comprising C 7 and/or C 6). They were found to contain MK-7 as the predominant menaquinone. The major polar lipids are phosphatidylethanolamine, an unidentified aminophospholipid and an unidentified lipid. Strains HMF5036, HMF5335 and HMF5405 exhibited the highest 16S rRNA gene sequence similarities of 91.8, 92.6 and 93.6 % to BUZ 2 and less than 88.7 % to other members of the family . Similarity values among the three isolates ranged from 94.9 to 96.6 %. Phylogenetic analysis based on the 16S rRNA gene sequences of the three isolates revealed that they formed a distinct clade within the family . The genome sizes of strains HMF5036, HMF5335 and HMF5405 were 6.8, 6.4 and 7.8 Mbp, and their DNA G+C contents were 54.9, 54.0 and 52.1 mol%, respectively. The average nucleotide identity, digital DNA–DNA hybridization and amino acid identity values between three isolates and BUZ 2 were 73.8–82.2, 19.6–25.4 and 75.0–87.5 %, respectively. These values were lower than the recommended threshold values for species delimitation. Based on the results of the phenotypic, genotypic, chemotaxonomic and phylogenetic investigations, three novel species, sp. nov., sp. nov. and sp. nov. are proposed. The type strains are HMF5036 (=KCTC 82476=NBRC 115092), HMF5335 (=KCTC 82477=NBRC 115093) and HMF5405 (=KCTC 82478=NBRC 115094), respectively.

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Keyword(s): Fibrella , forsythia , freshwater and rusty iron
© 2022 The Authors
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2022-05-13
2024-04-23
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Fibrella aquatilis sp. nov., Fibrella rubiginis sp. nov. and Fibrella forsythiae sp. nov., isolated from freshwater, rusty iron and forsythia flower
Int J Syst Evol Microbiol 72, 005366 (2022); https://doi.org/10.1099/ijsem.0.005366
/content/journal/ijsem/10.1099/ijsem.0.005366
/content/journal/ijsem/10.1099/ijsem.0.005366
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