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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 5

sp. nov., a marine bacterium isolated from cold spring area of South China Sea No Access

Abstract

A Gram-stain-negative, aerobic, non-motile, short-rod-shaped bacterium, designated strain hg1, was isolated from marine sediment within the cold spring area of South China Sea and subjected to a polyphasic taxonomic investigation. Colonies were circular and 1.0–2.0 mm in diameter, coral in colour, convex and smooth after growth on marine agar at 28 °C for 3 days. Strain hg1 was found to grow at 4–40 °C (optimum, 35–37 °C), at pH 6.5–9.0 (optimum, pH 7.5) and with 0–8 % (w/v) NaCl (optimum, 1.5–2 %). Chemotaxonomic analysis showed the sole respiratory quinone was MK-7, and the principal fatty acids are iso-C, summed feature 3 (C ω7 and/or C ω6), and iso-C. The major polar lipids are phosphatidylethanolamine, an unidentified phospholipid and five unidentified glycolipids. The DNA G+C content of strain hg1 was 39.6 mol% based on the genome sequence. The comparison of 16S rRNA gene sequence similarities showed that hg1 was closely related to DSM 15282 (98.6 % sequence similarity), MCCC 1F01099 (97.9 %) and DSM 17529 (97.2 %); it exhibited 97.0 % or less sequence similarity to the type strains of other species of the genus with validly published names. Phylogenetic trees reconstructed with the neighbour-joining, maximum-parsimony and maximum-likelihood methods based on 16S rRNA gene sequences showed that strain hg1 constituted a separate branch with . , , in a clade of the genus . OrthoANI values between strain hg1 and . , and were 94.3, 74.1, 73.2 %, respectively, and DNA–DNA hybridization values were 56.2, 18.5 and 18.3 %, respectively. Differential phenotypic properties, together with phylogenetic distinctiveness, demonstrated that strain hg1 is clearly distinct from recognized species of genus . On the basis of these features, we propose that strain hg1 (=MCCC 1K03570=KCTC 72111) represents a novel species of the genus with the name sp. nov.

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  • Accepted:
  • Published Online:
Keyword(s): Algoriphagus algorifonticola sp. nov. , marine bacterium and polyphasic taxonomy
Funding
This study was supported by the:
  • Science and Technology Basic Resources Investigation Program of China (Award 2017FY100300)
    • Principle Award Recipient: MinWu
  • Key R&D Program of Zhejiang (Award 2022C03010)
    • Principle Award Recipient: MinWu
© 2022 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005365
2022-05-23
2024-04-18
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Algoriphagus algorifonticola sp. nov., a marine bacterium isolated from cold spring area of South China Sea
Int J Syst Evol Microbiol 72, 005365 (2022); https://doi.org/10.1099/ijsem.0.005365
/content/journal/ijsem/10.1099/ijsem.0.005365
/content/journal/ijsem/10.1099/ijsem.0.005365
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