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Volume 72, Issue 5

sp. nov., isolated from the stem of in north-west China No Access

Abstract

A bacterial strain, designated YZJH907-2, was isolated from the stem of , collected from the southern edge of the Gurbantunggut desert, Xinjiang, PR China. Cells of strain YZJH907-2 were Gram-stain-positive, aerobic and rod-shaped. They formed white or colourless circular colonies with smooth convex surfaces. Strain YZJH907-2 grew at 4–50 °C (optimum, 28–30 °C), pH 7.0–10.0 (optimum, pH 8.0–9.0) and with 0–10 % (w/v) NaCl (optimum, 3–7 %). The genomic DNA G+C content of strain YZJH907-2 was 38.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that the strain was most closely related to DSM 485 (97.37 %), B16-24 (96.87 %) and LBB3 (96.71 %). Average nucleotide identity values between YZJH907-2 and DSM 485and LBB3 were 69.2 and 69.0 %, respectively. Digital DNA–DNA hybridization values of YZJH907-2 with DSM 485 and LBB3 were 19.6 and 20.4 %, respectively. The cell wall of strain YZJH907-2 contained -diaminopimelic acid, and the major and secondary isoprenoid quinones were MK-7 and MK-5, respectively. Results of fatty acids showed that anteiso-C, iso-C and C were the predominant cellular fatty acids. Two-dimensional thin-layer chromatography analysis indicated that the polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids and two unidentified glycolipids. Based on the genomic, phylogenetic and phenotypic analyses, strain YZJH907-2 represented a novel species of the genus , and thus the name sp. nov. is proposed. The type strain is YZJH907-2 (=CGMCC 1.18763=KCTC 43335).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacillus suaedae sp. nov. , endophyte , Gurbantunggut desert , haloalkaline ecosystem and halophyte
Funding
This study was supported by the:
  • open subject of the Key Laboratory of the Autonomous Region (Award 02017D0408)
    • Principle Award Recipient: Deng-DiAn
  • Scientific projects of colleges and universities in Xinjiang Autonomous Region (Award XJEDU2018I016)
    • Principle Award Recipient: Deng-DiAn
  • National Natural Science Foundation of China (Award 32061143043)
    • Principle Award Recipient: Wen-JunLi
  • National Natural Science Foundation of China (Award 31570109)
    • Principle Award Recipient: Deng-DiAn
  • National key R & D program young scientist project (Award 2021YFD1500300)
    • Principle Award Recipient: ShuangWang
© 2022 The Authors
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2024-04-25
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Bacillus suaedae sp. nov., isolated from the stem of Suaeda aralocaspica in north-west China
Int J Syst Evol Microbiol 72, 005362 (2022); https://doi.org/10.1099/ijsem.0.005362
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