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Abstract

An actinobacterium, designated strain EGI L10124, was isolated from saline lake sediment collected in Xinjiang province, PR China. The taxonomic position of the isolate was determined based on polyphasic taxonomic and phylogenomic analyses. Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that strain EGI L10124 formed a distinct clade with SYSU G02662, with a shared sequence identity of 95.2 %. The novel isolate could be distinguished from species in the genus by its distinct phenotypic, physiological and genotypic characteristics. The cells of strain EGI L10124 were aerobic, Gram-stain-positive and short rod-shaped. Optimal growth conditions of strain EGI L10124 on marine agar 2216 were registered at pH 8.0 at 37 °C. In addition, -diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The major respiratory quinone was MK-9 (H), while the major fatty acids were iso-C, C and C. The polar lipids included diphosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. Based on the genome sequence of strain EGI L10124, it appears that the G+C content of the novel isolate was 71.8 mol%. According to our data, strain EGI L10124 represents a new species of the genus , for which the name sp. nov. is proposed. The type strain of the proposed novel isolate is EGI L10124 (=CGMCC 1.19113=KCTC 49679).

Funding
This study was supported by the:
  • Xinjiang Uygur Autonomous Region regional coordinated innovation project (Award 2021E01018)
    • Principle Award Recipient: Wen-JunLi
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: Bao-ZhuFang
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2019FY100701)
    • Principle Award Recipient: Bao-ZhuFang
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2022-05-11
2022-07-06
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