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Volume 72, Issue 5

sp. nov., a moderately thermophilic chemotrophic bacterium unable to grow on hydrogen gas, isolated from hot spring microbial mats Open Access

Abstract

A novel thermophilic chemotrophic bacterium, strain SS56, was isolated from Nakabusa Hot Spring, Japan. The isolate was a rod-shaped (1.5–2.1×0.6–0.8 µm), Gram-stain-negative bacterium. The cells of this strain grew chemoheterotrophically under aerobic and anaerobic conditions. Autotrophic growth was observed with thiosulphate and elemental sulphur under aerobic conditions but not with H as the electron donor. Heterotrophic growth in the presence of O occurred on yeast extract, tryptone, polypeptone and organic acids. Strain SS56 used nitrite as an alternative electron acceptor under anaerobic chemoheterotrophic conditions. The isolate grew between 35 and 65 °C, with the optimum at 55 °C. The pH range for growth was pH 6.0–9.0; optimal growth occurred at pH 7.0–8.0. The 16S rRNA gene sequence of strain SS56 was 98.9% identical to that of TH-1. The draft genome sequence of 2401804 bp for strain SS56 gave values of 53.7% for digital DNA–DNA hybridization, 92.9% for average nucleotide identity and 93.6% for average amino acid identity compared with the genome sequence of 2223143 bp for TH-1. Based on the information described above, strain SS56 (=DSM 111892=JCM 34254) is proposed as the type strain of a novel species, sp. nov.

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Keyword(s): autotroph , heterotroph , Hydrogenophilales , Hydrogenophilalia , Hydrogenophilus , Hydrogenophilus thiooxidans and thermophile
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2022-05-11
2024-04-23
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Hydrogenophilus thiooxidans sp. nov., a moderately thermophilic chemotrophic bacterium unable to grow on hydrogen gas, isolated from hot spring microbial mats
Int J Syst Evol Microbiol 72, 005355 (2022); https://doi.org/10.1099/ijsem.0.005355
/content/journal/ijsem/10.1099/ijsem.0.005355
/content/journal/ijsem/10.1099/ijsem.0.005355
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