@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.005345, author = "Kong, Delong and Wang, Yan and Li, Qingqing and Zhou, Yanzheng and Jiang, Xu and Xing, Zhen and Wang, Zhiye and Ruan, Zhiyong", title = "Chryseobacterium subflavum sp. nov., isolated from soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2022", volume = "72", number = "5", pages = "", doi = "https://doi.org/10.1099/ijsem.0.005345", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005345", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "polyphasic taxonomy", keywords = "16S rRNA gene", keywords = "Chryseobacterium", eid = "005345", abstract = "A Gram-stain-negative, aerobic, non-motile, rod-shaped bacterium, designated LAMRS1T, was isolated from a soil sample collected in Hebei Province, PR China. Strain LAMRS1T was able to grow optimally in the presence of 0.5 % (w/v) NaCl, at pH 7.5 and at 30 °C. On the basis of 16S rRNA gene sequence analysis, strain LAMRS1T was closely related to members of the genus Chryseobacterium , with highest levels of sequence similarity to Chryseobacterium soli DSM 19298T (97.9 %), Chryseobacterium soldanellicola DSM 17072T (97.6%) and Chryseobacterium piperi CTMT (97.5 %). The average nucleotide identity and digital DNA–DNA hybridization values between LAMRS1T and the closely related species of C. soli DSM 19298T, C. soldanellicola DSM 17072T and C. piperi CTMT were 78.1, 78.2 and 80.7 %, and 21.7, 22.0 and 23.7 %, respectively. The draft genome sequence of LAMRS1T was 4.61 Mb, with DNA G+C content of 36.2 mol%. The major isoprenoid quinone was menaquinone-6 and iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1  ω6c and/or C16 : 1  ω7c) constituted the major cellular fatty acids. The main polar lipids were phosphatidylethanolamine, four aminolipids, three glycolipids and seven unidentified lipids. On the basis of evidence presented in this study, strain LAMRS1T represents a novel species of the genus Chryseobacterium , for which the name Chryseobacterium subflavum sp. nov. is proposed. The type strain is LAMRS1T (=JCM 33868T=KCTC 72823T).", }