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Volume 72, Issue 5

gen. nov., sp. nov., a member of the family isolated from seawater of the Indian Ocean and intertidal zone No Access

Abstract

Two Gram-stain-negative, facultatively anaerobic, non-motile, rod-shaped bacteria, strains h42 and ALG8, were isolated individually from the Indian Ocean and intertidal zone of Zhoushan, China. The results of 16S rRNA gene sequence analysis showed that the sequence similarity between strains h42 and ALG8 was 99.7 %, and the closest related strains were C7 (97.77 and 97.62 %) and GYSW-23 (95.31 and 95.45 %). Phylogenetic analysis based on 16S rRNA gene sequences shows that these two novel strains belong to a distinct new lineage of the family in the order . The average nucleotide identity and DNA–DNA hybridization values between the two novel strains and C7 and GYSW-23 were 72.73–78.15 % and 19.70–20.80 %, respectively. The DNA G+C content of strains h42 and ALG8 was 62.36 % and 62.17 mol %. The major fatty acids (>10 %) in strain h42 were C, C cyclo 8 and summed feature 8 (C 6 and/or C 7), and in strain ALG8 were C cyclo 8 and summed feature 8 (Cω6 and/or C 7). The predominant isoprenoid ubiquinone of the two novel strains was Q-10; their major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified glycolipids, an unidentified aminoglycolipid, an unidentified phospholipid and an unidentified lipid. Based on the results of the morphological, physiological, chemotaxonomic and phylogenetic analysis of these two strains, a novel species of a new genus in the family is proposed, named as gen. nov., sp. nov. The type strain and non-type strain of are h42 (=KCTC 72875=CGMCC 1.17849=MCCC 1K04735) and ALG8 (=KCTC 82194=MCCC 1K04733).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): offshore , open sea , phylogenetic analysis , Pontibrevibacter nitratireducens and Rhodobacteraceae
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 91851114)
    • Principle Award Recipient: Xue-WeiXu
  • National Key R&D Program of China (Award 2018YFC0310704)
    • Principle Award Recipient: HongCheng
© 2022 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005341
2022-05-06
2024-04-19
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Pontibrevibacter nitratireducens gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from seawater of the Indian Ocean and intertidal zone
Int J Syst Evol Microbiol 72, 005341 (2022); https://doi.org/10.1099/ijsem.0.005341
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