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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 4

gen. nov., sp. nov., a new member of the family isolated from soil No Access

Abstract

A Gram-stain-negative, non-motile, moderately halophilic and facultatively anaerobic bacterium, designated YR4-1, was isolated from a saline-alkali and sorghum-planting soil sample collected in Dongying, Shandong Province, PR China. Growth occurred at 28–45 °C with the presence of 4.0–20.0 % (w/v) NaCl and pH 6.0–9.0. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that YR4-1 shared the highest similarity of 92.1–92.4 % with the valid published species of . The isolate formed a separate clade at the genus level in recently described family . The draft genome of strain YR4-1 is 3.83 Mbp long with 44.0 mol% G+C content. The strain possesses several genes involved in the osmotic stress response mechanism and diverse metabolic pathways, probably for the living in saline environment. This may lead to a better understanding of the underrepresented lineage. The major menaquinone was MK-7. The main polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipids, aminophosphoglycolipid, one glycolipid, and four unidentified lipids. The predominant cellular fatty acids were iso-C (35.7 %) and anteiso-C (33.5 %). On the basis of its phenotypic, chemotaxonomic and phylogenetic features, strain YR4-1 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is YR4-1 (=CGMCC 1.17777=KCTC 72795).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Halalkalibacterium roseum , novel genus and polyphasic taxonomy
Funding
This study was supported by the:
  • Ministry of Science and Technology of the People's Republic of China (Award No. 2019FY100700)
    • Principle Award Recipient: DuohongSheng
  • National Natural Science Foundation of China (Award Nos. 32070030 and 31670076)
    • Principle Award Recipient: Yue-zhongLi
  • Ministry of Science and Technology of the People's Republic of China (Award No. 2017FY100302)
    • Principle Award Recipient: Yue-zhongLi
© 2022 The Authors

Erratum

An erratum has been published for this content:
Corrigendum: gen. nov., sp. nov., a new member of the family isolated from soil
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Halalkalibacterium roseum gen. nov., sp. nov., a new member of the family Balneolaceae isolated from soil
Int J Syst Evol Microbiol 72, 005339 (2022); https://doi.org/10.1099/ijsem.0.005339
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