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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 5

sp. nov., a catalase-negative new member of the genus, isolated from Anatolian Ground Squirrel () in Turkey No Access

Abstract

Eleven Gram-negative, curved and S-shaped, oxidase activity positive, catalase activity negative bacterial isolates recovered from faeces of Anatolian ground squirrel () in the city of Kayseri, Turkey, were subjected to a polyphasic taxonomic study. Results of a genus-specific PCR revealed that these isolates belonged to the genus . The 16S rRNA gene sequence analysis revealed that the 11 isolates had over 99 % sequence identity with each other and were most closely related to CMRI H02 with 97.0–97.1 % identity levels and they formed a novel phylogenetic line within the genus . Faydin-H64 and Faydin-H70 strains were subjected to A and A gene and whole genome sequence analyses. These two strains formed separate phylogenetic clades, divergent from other known species. The DNA G+C content and genome size of the strain Faydin-H70 were 35.3 mol% and 1.7 Mb, respectively. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain Faydin-H70 and its close phylogenetic neighbour ATCC BAA-430 were determined as 81.7 and 34.9 %, respectively. Pairwise sequence comparison showed that it was closely related to CMRI H02 however it shared the highest ANI and dDDH values with ATCC BAA-430. The data obtained from the polyphasic taxonomy approach, including phenotypic characterization and whole-genome sequences, revealed that these strains represent a novel species within the genus , for which the name sp. nov., is proposed with Faydin-H70 as the type strain (=DSM 112556=LMG 32335).

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Keyword(s): Anatolian ground squirrel , feces , Helicobacter , novel species and overall genome-related index
© 2022 The Authors
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2022-05-04
2024-04-24
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Helicobacter turcicus sp. nov., a catalase-negative new member of the Helicobacter genus, isolated from Anatolian Ground Squirrel (Spermophilus xanthoprymnus) in Turkey
Int J Syst Evol Microbiol 72, 005338 (2022); https://doi.org/10.1099/ijsem.0.005338
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