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Volume 72, Issue 4

sp. nov., a novel species of the family isolated from plastic waste Open Access

Abstract

Strain NGK35 is a motile, Gram-stain-negative, rod-shaped (1.0–2.1 µm long and 0.6–0.8 µm wide), aerobic bacterium that was isolated from plastic-polluted landfill soil. The strain grew at temperatures between 6 and 37 °C (optimum, 28 °C), in 0–10 % NaCl (optimum, 1 %) and at pH 6.0–9.5 (optimum, pH 7.5–8.5). It was positive for cytochrome c oxidase, catalase as well as HS production, and hydrolysed casein and urea. It used a variety of different carbon sources including citrate, lactate and pyruvate. The predominant membrane fatty acids were C cis9 and C, followed by C cyclo and C cis11. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine, followed by diphosphatidyglycerol. The only quinone was ubiquinone Q-8. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NGK35 belongs to the genus (family ), appearing most closely related to CCUG 53761A (96.90 %) and ABC02-12 (96.94 %). The genomic DNA G+C content of strain NGK35 was 52.1 mol %. Genome-based calculations (genome-to-genome distance, average nucleotide identity and DNA G+C content) clearly indicated that the isolate represents a novel species within the genus . Based on phenotypic and molecular characterization, strain NGK35 can clearly be differentiated from its phylogenetic neighbours establishing a novel species, for which the name sp. nov. is proposed. The type strain is NGK35 (=DSM 113270=NCCB 100854).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bacteria , Paenalcaligenes niemegkensis , plastic pollution and terrestrial plastisphere
Funding
This study was supported by the:
  • Helmholtz Recruiting Initiative (Award I-044-16-01)
    • Principle Award Recipient: VladimirRoddatis
  • Bundesministerium für Bildung und Forschung (Award 02WPL1449D)
    • Principle Award Recipient: DirkWagner
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2022-04-25
2024-04-18
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Paenalcaligenes niemegkensis sp. nov., a novel species of the family Alcaligenaceae isolated from plastic waste
Int J Syst Evol Microbiol 72, 005333 (2022); https://doi.org/10.1099/ijsem.0.005333
/content/journal/ijsem/10.1099/ijsem.0.005333
/content/journal/ijsem/10.1099/ijsem.0.005333
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