@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.005330, author = "Li, Qing and Shen, Taiming and Dong, Yingying and Liu, Yuhui and Nong, Jieliang and Pan, Jiayuan and Fu, Bei and Xie, Qinglin", title = "Clostridium weizhouense sp. nov., an anaerobic bacterium isolated from activated sludge of petroleum wastewater", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2022", volume = "72", number = "4", pages = "", doi = "https://doi.org/10.1099/ijsem.0.005330", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005330", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "taxonomy", keywords = "new species", keywords = "anaerobic bacteria", keywords = "Clostridium", eid = "005330", abstract = "A Gram-stain-positive, anaerobic, spore-forming, rod-shaped (0.4–0.6 µm×2.5–3.2 µm), flagellated bacterium, designated strain YB-6T, was isolated from activated sludge of an anaerobic tank at Weizhou marine oil mining wastewater treatment plant in Beihai, Guangxi, PR China. The culture conditions were 25–45 °C (optimum, 37 °C), pH 4–12 (pH 7.0) and NaCl concentration of 0–7 % w/v (0%). Strain YB-6T grew slowly in petroleum wastewater and removed 68.2 % of the total organic carbon in petroleum wastewater within 10 days. Concentrations of naphthalene, anthracene and phenanthrene at an initial concentration of 50 mg l−1 were reduced by 32.8, 40.4 and 14.6 %, respectively, after 7 days. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain YB-6T belongs to Clostridium cluster I and is most closely related to Clostridium uliginosum CK55T (98.5 % similarity). The genome size of strain YB-6T was 3.96 Mb, and the G+C content was 26.5 mol%. The average nucleotide identity value between strain YB-6T and C. uliginosum CK55T was 81.9 %. The major fatty acids in strain YB-6T were C14 : 0 FAME, C16 : 0 FAME and summed feature 4 (unknown 14.762 and/or C15 : 2 FAME). The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five unidentified aminophospholipids, one unidentified glycolipid and one unidentified aminolipid. Diaminopimelic acid was not detected in the strain YB-6T cell walls. Whole-cell sugars mainly consisted of ribose and galactose. Based on the results of phenotypic and genotypic analyses, strain YB-6T represents a novel species of the genus Clostridium , for which the name Clostridium weizhouense sp. nov. is proposed. The type strain is YB-6T (=GDMCC 1.2529T=JCM 34754T).", }