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Volume 72, Issue 3

sp. nov., an ammonium-tolerant acetate-producing bacterium isolated from a biogas system Open Access

Abstract

An anaerobic bacterial strain, designated AMB_01, recovered from mesophilic propionate enrichment of a high-ammonia biogas digester, was characterised using phenotypic and molecular taxonomic methods. Cells of AMB_01 are coccus-shaped and often occur arranged as diplococci or sarcina. Growth occurred at 20–45 °C, initial pH 5.5–8.5 and with up to 0.7 M NHCl, with optimum growth at 37–42 °C and pH 8.0. AMB_01 achieved high cell density and highest acetate production when grown on carbohydrates, including monomers, disaccharides and polysaccharides, such as glucose, maltose, cellobiose and starch. The strain was also able to use amino acids and some organic acids and alcoholic compounds for growth. Acetate was formed as the main product and yeast was not required for growth. The major cellular fatty acids were summed feature 4 (iso-CI and/or anteiso-CB), Cω7, C, C and summed feature 3 (Cω7 and/or iso-C 2OH). The highest 16S rRNA gene sequence similarity found was with (96.6 %), within the family , phylum Bacillota (Firmicutes). The genomic DNA G+C content was 29.0 mol%. An almost complete set of genes for the acetyl-CoA pathway was found. Genome comparisons between AMB_01 and close relatives showed highest digital DNA–DNA hybridisation to (23 %), highest average nucleotide identity with genome nucleotide and amino acid sequences to (72 and 73 %, respectively) and highest average nucleotide identity (87 %) with , indicating that AMB_01 represents a novel species. Analysis of genomic, chemotaxonomic, biochemical and physiological data confirmed that strain AMB_01 represents a novel species, for which the name sp. nov. is proposed. The type strain is AMB_01 (=DSM 110247=JCM 39107 ).

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  • Accepted:
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Keyword(s): ammonia-tolerant , anaerobic digestion , hydrogen-producing and Peptoniphilaceae
Funding
This study was supported by the:
  • Swedish Research Council Formas (Award 2018-01341)
    • Principle Award Recipient: SchnürerAnna
  • National Natural Science Foundation of China (Award 51778616)
    • Principle Award Recipient: WeiQiao
  • National Natural Science Foundation of China (Award 51778616)
    • Principle Award Recipient: ShaojieBi
  • Sveriges Lantbruksuniversitet
    • Principle Award Recipient: AbhijeetSingh
  • Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (Award 2012-808)
    • Principle Award Recipient: MariaWesterholm
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2022-03-21
2024-04-24
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Miniphocaeibacter halophilus sp. nov., an ammonium-tolerant acetate-producing bacterium isolated from a biogas system
Int J Syst Evol Microbiol 72, 005328 (2022); https://doi.org/10.1099/ijsem.0.005328
/content/journal/ijsem/10.1099/ijsem.0.005328
/content/journal/ijsem/10.1099/ijsem.0.005328
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