1887

Abstract

A novel pale white-pigmented bacterial strain designated YC-7-48 was isolated from activated sludge in China. Cells of the strain, which grew at 15–37 °C (optimum at 30 °C) and pH 6.0–9.0 (optimum at 7.0), were Gram-stain-negative, rod-shaped and motile. Strain YC-7-48 had 97.4–97.1% 16S rRNA gene sequence similarity to type strains of eight species in the genera , , , and of the family . Phylogenetic analysis based on 16S rRNA gene sequencing placed the strain on a separate branch in the genus and showed that it exhibited 97.4, 97.3 and 96.6% similarity to EBR-8-1, BN9 and 17-4A, respectively. The genome size of strain YC-7-48 was 3202438 bp, with 54.3 mol% G+C content. According to the genome analysis, YC-7-48 encodes several heavy metal resistance proteins and enzymes related to the metabolism of nicotine and aromatic compounds. The results of digital DNA–DNA hybridization and average nucleotide identity analyses based on whole genome sequences between strain YC-7-48 and the closely related strains indicated that the strain represented a new species of the genus . The chemotaxonomic results identified Q-8 as the predominant respiratory quinone, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and two unidentified aminolipids as the major polar lipids, and C (27.4 %), C cyclo (22.0 %), C (11.7 %) and C cyclo 8 (9.5 %) as the major fatty acids. Thus, based on morphological, chemotaxonomic and phylogenetic characterization and genomic data, we proposed that the isolate is a representative of a novel species named sp. nov., with the type strain YC-7-48 (=CGMCC 1.17466=KACC 21349).

Funding
This study was supported by the:
  • Doctoral Scientific Research Foundation of Yancheng Teachers University (Award 72071671003C)
    • Principle Award Recipient: LiYao
  • National Natural Science Foundation of China (Award 31700096)
    • Principle Award Recipient: LiYao
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2022-04-22
2022-05-22
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