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Abstract

Two Gram-staining-negative, aerobic, rod-shaped bacteria NNCM1 and NNCM2 were isolated from the scleractinian coral . NNCM1 grew with 0.5–12 % (w/v) NaCl (optimum, 3–6 %), at 18–37 °C (optimum, 28 °C) and at pH 6.0–10.0 (optimum, 7.0–8.0). NNCM2 grew with 0.5–10 % (w/v) NaCl (optimum, 2 %), at 18–37 °C (optimum, 28 °C) and at pH 6.5–9.0 (optimum, 7.0). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that NNCM1 formed a lineage within the genus of the family Algiphilaceae, and it was distinct from the most closely related species DG1253, with a 16S rRNA gene sequences similarity of 97.05 %. NNCM2 formed a lineage within the family Rhodobacteraceae, and it was distinct from the closely related genera CAU 1123, D4M1 and MME-001 with 93.41, 92.78 and 91.09% identities, respectively. The major respiratory quinone was Q-8 and Q-10 for NNCM1 and NNCM2, respectively. The predominant fatty acids (more than 10 %) were summed feature 8 (39.4 %) and C (19.4 %) for NNCM1 and summed feature 8 (62.8 %) and C (12.4 %) for NNCM2. The DNA G+C contents of NNCM1 and NNCM2 were 63.3 and 63.4 mol% respectively. The polar lipids of NNCM1 comprised one diphosphatidylglycerol, one phosphatidylethanolamine, one phosphatidylglycerol and one unknown polar lipid, while those of NNCM2 comprised one phosphatidylethanolamine, one phosphatidylglycerol, one aminolipid and four unknown polar lipids. Phenotypic characteristics (physiological, biochemical and chemotaxonomic) also supported the taxonomic novelty of the two isolates. Thus, NNCM1 is considered to represent a novel species within genus , for which the name sp. nov. is proposed. The type strain is NNCM1 (=KCTC 82966=MCCC 1K06445). NNCM2 represents a novel genus and species within the family Rhodobacteraceae, for which the name gen. nov. sp. nov. is proposed. The type strain is NNCM2 (=KCTC 82967=MCCC 1K06408).

Funding
This study was supported by the:
  • Guangdong Key Area R & D Program Project (Award 2020B1111030002)
    • Principle Award Recipient: BaohuaXiao
  • Shenzhen Science and Technology R&D Fund (Award JCYJ20200109144803833)
    • Principle Award Recipient: BaohuaXiao
  • Shenzhen Science and Technology R&D Fund (Award KJYY20180213182720347)
    • Principle Award Recipient: BaohuaXiao
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2022-04-19
2022-05-18
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