sp. nov., an antimicrobial producing actinobacterium isolated from soil under black Gobi rocks No Access

Abstract

A novel actinomycete, strain 1_25, was isolated from soil under a black Gobi rock sample from Shuangta, PR China, and characterized using a polyphasic taxonomic approach. The results of comparative analysis of the 16S rRNA gene sequences indicated the 1_25 represented a member of the genus . Chemotaxonomic data revealed that 1_25 possessed MK-9(H) as the major menaquinone. The cell wall contained -diaminopimelic acid (-DAP) and the whole-cell sugar pattern consisted of ribose, glucose and galactose. Major fatty acid methyl esters were observed to be iso-C (23.6 %), and anteiso-C (10.4 %). The genomic DNA G+C content of 1_25 was 69 mol %. The results of phylogenetic analysis based on 16S rRNA gene sequence indicated that 1_25 had high sequence similarity with 172205 (98.1 %), TRM 66187 (98 %), and JCM4580 (98 %). In addition to the differences in phenotypic characters, the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between 1_25 and closely related species were below the recommended threshold values for assigning strains to the same species. The fermentation product of 1_25 in ISP2 had an inhibitory effect on . On the basis of these genotypic and phenotypic characteristics, strain 1_25 (=JCM 34936=GDMCC 4.216) represents a novel species of the genus , for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • the Scientific Project of Gansu Province, China (Award 20JR5RA548)
    • Principle Award Recipient: yingwen
  • the Scientific Project of Gansu Province, China (Award 18JR2TA019)
    • Principle Award Recipient: yingwen
  • the Scientific Project of Gansu Province, China (Award 20YF3WA007)
    • Principle Award Recipient: yingwen
  • the National Key R&D Program of China (Award 2019YFE0121100)
    • Principle Award Recipient: TuoChen
  • The National Natural Science Foundation of China (Award 31870479)
    • Principle Award Recipient: WeiZhang
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2022-03-23
2024-03-28
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